搜索结果: 1-15 共查到“知识库 家畜寄生虫学”相关记录485条 . 查询时间(0.806 秒)
硫酸化酵母葡聚糖对鸡球虫病的防治效果
硫酸化酵母葡聚糖 球虫 肠道菌 药物滥用
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2021/5/8
旨在探讨硫酸化酵母葡聚糖（sGSC）对人工感染鸡柔嫩艾美耳球虫（Eimeria tenella）病的防治效果。采用笼饲法，将200只鸡随机分为A组（4 mg·kg-1）、B组（16 mg·kg-1）、C组（64 mg·kg-1）、D组（感染对照组）和E组（健康对照组）。感染球虫前3 d开始，给A、B、C组鸡每天按体重饮水喂上述各浓度的sGSC，给D、E组的鸡喂纯水（RO水），持续至试验结束。试验过...
柔嫩艾美耳球虫延伸因子1α特性与功能初步分析
柔嫩艾美耳球虫 延伸因子1α 特性分析 入侵
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2021/3/8
鸡球虫病在世界范围内对家禽业具有重大的经济影响，导致高死亡率、低增长、低生产力和高防治成本。由于球虫耐药性问题严峻，球虫病的防治重点倾向于研究方便安全，并且与活疫苗相比更容易生产的亚单位疫苗。因此，迫切需要新的方法来有效地控制球虫病，包括寻找特定的分子靶标。本文以柔嫩艾美耳球虫孢子化卵囊cDNA第一链为模板，扩增了延伸因子1α（EtEF1α）的ORF序列，生物信息学分析显示，该基因编码450个氨基...
1种检测弓形虫急性感染的夹心ELISA方法
弓形虫急性感染 GRA1单克隆抗体 循环抗原 双抗体夹心ELISA
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2021/3/8
弓形虫（Toxoplasma gondii）是一种人畜共患机会性致病原虫，其急性感染可导致宿主产生明显的临床症状和严重的病理损伤。弓形虫致密颗粒蛋白1（dense granuleprotein 1，GRA1）是一种良好的诊断抗原，也是弓形虫急性感染的标志物循环抗原（circulating antigen，CAg）的重要组分。本研究利用TgGRA1单克隆抗体建立双抗体夹心ELISA方法，为急性弓形虫...
旨在探究大熊猫粪便中微生物组成以及耐药基因和寄生虫的真实情况。采集6只健康成年大熊猫的新鲜粪便，利用宏转录组学测序技术分析大熊猫粪便微生物组成及功能、耐药基因种类、丰度和寄生虫的组成，探讨优势细菌与耐药基因的相关性。结果表明，大熊猫粪便内微生物种类多样，包括细菌、真菌和病毒等，但以细菌为主。在门水平上，细菌类群中厚壁菌门（Firmicutes）和变形菌门（Proteobacteria）为优势菌门，...
猫源胎儿三毛滴虫的形态学观察及分子鉴定
猫 胎儿三毛滴虫 分离鉴定
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2021/3/12
旨在进一步鉴定从1例长期慢性腹泻猫粪便中新分离到的1株毛滴虫，分别对其进行了分离与体外培养、形态学观察及18S rRNA基因序列相似性分析。形态学观察结果显示，分离株毛滴虫呈梨形或纺锤形，且具有3根前鞭毛和1根后鞭毛，为胎儿三毛滴虫形态结构。18S rRNA基因序列与GenBank中三毛滴虫基因序列比对及系统进化树结果显示，分离的猫源毛滴虫序列与胎儿三毛滴虫MH400076.1处于同一分支，且相似...
旨在克隆、表达马泰勒虫（Theileria equi）和驽巴贝斯虫（Babesia caballi）半胱氨酸蛋白酶（cystein protease，CP）基因，并对其进行生物信息学预测和分析。提取马泰勒虫和驽巴贝斯虫的DNA，以此为模板扩增目的基因。利用克隆技术和原核表达系统克隆表达CP，用尿素透析法进行纯化。通过Dot blot验证其与相应阳性血清特异性反应。应用MAGA软件和Prot Par...
细粒棘球绦虫原头蚴microRNA表达谱分析
细粒棘球绦虫 microRNA 原头蚴 表达谱
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2019/1/17
旨在解析miRNA在细粒棘球绦虫原头蚴中的表达谱特性，为进一步揭示miRNA在细粒棘球绦虫原头蚴发育中的作用及调控机制提供理论依据。从患病绵羊肝无菌采集细粒棘球绦虫原头蚴，提取总RNA，构建原头蚴miRNA文库，利用RNA sequencing技术进行测序分析。结果表明，本研究从细粒棘球绦虫原头蚴中分离得到109个已知的miRNA分子，同时得到189个新的miRNA发夹前体分子。靶基因的预测结果显...
The occurrence of pathogens in Rhipicephalus microplus ticks from cattle in Madagascar
African ticks Anaplasma Babesia Borrelia Ehrlichia zebu Boophilus southern cattle tick
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2016/10/14
Rhipicephalus microplus is one of the most important ectoparasites of cattle in tropical and subtropical regions. In ticks collected from cattle the pathogens Babesia bovis, Anaplasma spp. and Ehrlich...
宿主细胞F-actin聚集在顶复门原虫入侵过程中的作用研究
顶复门原虫 宿主细胞 F-actin聚集 入侵
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2016/3/30
病原入侵宿主细胞，由宿主细胞肌动蛋白（F-actin）聚集引起的细胞骨架重排起着极其重要的作用，本文主要就宿主细胞整合素介导的宿主细胞F-actin聚集重排在顶复门原虫入侵过程中的作用进行系统的阐述，对解析顶复门原虫的入侵机制及以此研制新型寄生虫病药物和疫苗具有非常重要的意义。
Prevalence of Trichinella spp. antibodies in wild boars (Sus scrofa) and domestic pigs in Korea
Trichinella spp. trichinellosis seroprevalence ELISA wild boar domestic pig
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2015/4/30
Trichinellosis is a parasitic zoonosis that is of importance to public health; human trichinellosis usually occurs when improperly cooked pork or wild animal meat is consumed. The purpose of this stud...
Effects of Neospora caninum on reproductive performance and the efficacy of treatment with a combination of sulphadiazine-trimethoprim and toltrazuril: a longitudinal field study
neosporosis dairy cow abortion fertility treatment
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2015/4/17
This study was designed to determine the prevalence of Neospora caninum, the effect of infection on abortion and fertility parameters and the efficacy and outcomes with combination treatment in a dair...
First molecular characterisation of Giardia duodenalis infection in dairy goats in Brazil
goat Giardia duodenalis molecular characterisation genotyping
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2015/4/17
The aim of this study was to perform the first molecular genotyping of Giardia duodenalis from goats in Brazil, in order to assess the risk for zoonotic transmission. Samples were collected from two d...
First report of infestation by a parasitic copepod (Pennella balaenopterae) in a harbour porpoise (Phocoena phocoena) from the Aegean Sea: a case report
copepod Pennella balaenopterae harbour porpoise ectoparasite southern Aegean Sea
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2015/4/17
An adult, female harbour porpoise (Phocoena phocoena relicta) was found stranded on the southern Aegean Sea coast of Turkey. Thirteen holes made by copepods were observed on the lateral sides of the p...
Evidence of Neospora caninum exposure among native Korean goats (Capra hircus coreanae)
neosporosis seroprevalence native Korean goats ELISA Capra hircus coreanae
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2015/4/17
Neospora caninum is a protozoan parasite that causes abortion in ruminants, including goats. The objective of the present study was to determine the seroprevalence of N. caninum in native Korean goats...
Description of peritoneal cavity dirofilariosis caused by Dirofilaria immitis (Filarioidea: Onchocercidae) in a dog: a case report
dog Dirofilaria immitis peritoneal cavity
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2015/4/17
We describe Dirofilaria immitis occurring in the peritoneal cavity of a dog from Korea. Two worms were found in the fat of the umbrical cord area during operation for an umbrical cord hernia in a dog ...