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中国科学院武汉植物园“巫山淫羊藿及其易混物种的EST-SSR分子鉴定方法”获国家发明专利授权
巫山淫羊藿 混物种 EST-SSR分子 专利授权
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2022/10/26
2022年9月28日，从国家知识产权局获悉，武汉植物园药用植物资源学科组“巫山淫羊藿及其易混物种的EST-SSR分子鉴定方法”获国家发明专利授权。专利号:ZL201910434725.4，发明人：张燕君，于东悦，梁琼，王瑛，杨路路。
目的 基于DNA条形码技术建立人参与西洋参种子的分子鉴别方法。方法 采用生药鉴定方法研究其性状、显微特征，并结合DNA条形码技术，基于中药材DNA条形码数据库，通过ITS2序列比对、遗传距离比较和构建临接（NJ）系统发育树，对人参与西洋参的种子进行鉴别研究。结果 人参与西洋参种子的种内遗传距离分别小于种间遗传距离，系统发育树图中各物种均聚为一支，来自9个产地的42份人参及西洋参的种子均为正品，且容...
分析来士普联合认知行为治疗对脑卒中后抑郁(PSD)的疗效。收集200例PSD患者并随机分为空白对照组,对照组1,对照组2及观察组。空白对照组不进行任何治疗,对照组1实施认知行为治疗,对照组2实施来士普治疗,观察组在对照组2用药基础上进行认知行为治疗。四组患者分别与治疗前24 h及治疗12周后进行汉密顿抑郁量表(HAMD)、神经功能缺损程度量表(NIHSS)和改良Banhel指数(MBI)评分。治疗...
分别采用浸渍法和药膜法测定了辛硫磷对枸杞木虱不同发育期若虫和成虫的毒力，同时测定了辛硫磷对不同龄期枸杞木虱羧酸酯酶（CarE）活性的抑制作用及酶的动力学常数。结果表明：辛硫磷对枸杞木虱的LC50值随若虫龄期的增大而升高，至成虫期再相对降低，其对5龄若虫的LC50值为0.995 mg/L，是对1龄若虫LC50值（0.047 mg/L）的21.2倍。辛硫磷对不同龄期枸杞木虱羧酸酯酶活性的抑制作用I50...
为明确氟唑菌酰胺和吡唑醚菌酯在芒果上的残留行为，于2012和2013年在中国广东省和广西自治区进行了氟唑菌酰胺和吡唑醚菌酯在芒果上的田间残留及消解动态试验，建立了芒果中氟唑菌酰胺及吡唑醚菌酯残留量的高效液相色谱检测方法。样品用丙酮提取，乙酸乙酯液-液分配萃取，弗罗里硅土柱层析净化，高效液相色谱-二级管阵列紫外检测器检测，外标法定量。结果表明：氟唑菌酰胺和吡唑醚菌酯在芒果上的消解半衰期分别为7.2～...
Negative Cross-Resistance of Acetolactate Synthase Inhibitor–Resistant Kochia (Kochia scoparia) to Protoporphyrinogen Oxidase– and Hydroxyphenylpyruvate Dioxygenase–Inhibiting Herbicides
ALS-inhibitor resistance herbicide resistance pleiotropic effect target-site mutation
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2015/12/23
This greenhouse experiment examined the response of homozygous susceptible and acetolactate synthase (ALS) inhibitorresistant plants from six Canadian kochia accessions with the Pro197 or Trp574 mutat...
Meligethes aeneus (Coleoptera: Nitidulidae) resistance to lambda-cyhalothrin in the Czech Republic in 2012 and 2013
pollen beetles pyrethroid resistance esteric pyrethroids adult vial tests IRAC
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2015/7/28
Susceptibility of Meligethes aeneus populations from the Czech Republic and Slovakia was tested with lambda-cyhalothrin (93 populations in 2012, 82 populations in 2013) using IRAC method No. 011 Versi...
Interaction of two neonicotinoid insecticides and Lr genes focusing wheat growth and residues
insecticide residues wheat protection Lr 20 Lr 29
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2015/7/28
Seedlings of ten Lr near-isogenic lines (NIL) and four wheat lines with enhanced resistance to Puccinia triticina were treated with overdosed neonicotinoid insecticides. The enzyme of Lr 20 gene accel...
Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test:role of opioidergic and histaminergic systems
Bunium persicum antinociception writhing test mouse
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2015/4/30
Bunium persicum (Boiss.) is an economically important medicinal plant growing wildly in arid regions in Iran. The fruit of B. persicum is widely used in traditional Iranian medicine to control colic p...
Modulation of Polyamine Catabolism in Pea Seedlings by Calcium during Salinity Stress
Pisum sativum calcium ions potassium ions
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2015/3/9
The relation of polyamine catabolism in the response of Pisum sativum to salinity stress was investigated. Pea
seedlings were grown in increasing concentrations of Na+ or K+ or at different concentr...
Evaluation of Various Plant Extracts against the Early Blight Disease of Tomato Plants under Greenhouse and Field Conditions
Alternaria solani Neem Sweat Basil Eucalyptus
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2015/3/9
The antimicrobial activity of six plant extracts from ocimum basilicum (Sweat Basil), Azadirachta indica (Neem),
Eucalyptus chamadulonsis (Eucalyptus), Datura stramonium (Jimsonweed), Nerium oleande...
直接竞争酶联免疫吸附分析法测定小白菜中溴氰菊酯的残留量
直接竞争酶联免疫吸附分析 高效液相色谱 小白菜 溴氰菊酯 残留
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2016/7/27
应用作者所在实验室自制的、对溴氰菊酯具有特异性亲和力的多克隆抗体和酶标半抗原,建立了包被抗体-酶标半抗原直接竞争酶联免疫吸附分析法(DC-ELISA)。小白菜样品经超声提取、浓缩和10%甲醇定容后采用DC-ELISA法测定,并采用高效液相色谱法进行验证。结果表明:在抗体包被浓度为2.0 mg/L、辣根过氧化物酶(HRP)标记溴氰菊酯半抗原稀释2.0×105倍、包被介质为pH 7.2 PB、反应介质...
Bacillus pumilus – a new phytopathogen of Scots pine
Pinus sylvestris bacteriosis phytopathogenic bacteria soft rot
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2015/5/6
Large bleeding lesions on stem and branches of pine trees, wet black spots and depressed cancer wounds of different sizes at the base of knots, dying off the bark around knots and sapwood exposure ove...
Chemically Modified Chitosans as Antimicrobial Agents against Some Plant Pathogenic Bacteria and Fungi
chemically modified chitosans antibacterial activity
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2015/3/5
The antimicrobial activity of eight chemically modified chitosans against plant pathogenic bacteria of crown gall
disease agrobacterium tumefaciens and soft mould disease Erwinia carotovora and fung...
New Findings and Vegetative Compatibility Groups of Cryphonectria parasitica (Murrill) M. E. Barr in the Czech Republic
chestnut blight Cryphonectria parasitica
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2015/3/5
This study provides new information on the distribution of the chestnut blight agent Cryphonectria parasitica
and its vegetative compatibility groups (VCGs) in the Czech Republic. This study has rev...