搜索结果: 1-12 共查到“知识库 医学 VacA”相关记录12条 . 查询时间(0.181 秒)
Evaluation of Helicobacter pylori vacA and cagA Genotypes and Correlation With Clinical Outcome in Patients With Dyspepsia in Hamadan Province,Iran
Helicobacter Pylori VacA cagA Genotyping
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2015/10/15
Background: Helicobacter pylori is known to be a causative agent of chronic active gastritis, peptic ulcer and gastric cancer in human. Diverse genotypes of H. pylori strains have different virulence ...
Genetic Analysis of cagA and vacA Genes in Helicobacter Pylori Isolates and Their Relationship with Gastroduodenal Diseases in the West of Iran
Helicobacter Pylori VacA Protein Helicobacter Pylori CagA protein Helicobacter Pylori
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2015/10/12
Background: Helicobacter pylori have different virulence factors which are associated with several gastroduodenal diseases; however, this association is variable in different geographical regions. Dat...
Genetic Analysis of cagA and vacA Genes in Helicobacter Pylori Isolates and Their Relationship with Gastroduodenal Diseases in the West of Iran
Helicobacter Pylori VacA Protein Helicobacter Pylori CagA protein Helicobacter Pylori
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2015/9/30
Background: Helicobacter pylori have different virulence factors which are associated with several gastroduodenal diseases; however, this association is variable in different geographical regions. Dat...
Studying the Importance of VacA Gene of Helicobacter pylori in Identifying the Pathogenicity of Strains by Comparing It with the Disease Status of the Subjects
Helicobacter pylori 16S rRNA VacA Rapid Urease Test Culture
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2013/1/30
Introduction: H. pylori is a primary pathogen isolated by Warren and Marshall in 1983. They called it as Campylobacter Pylori and in 1989 Goodwin et al. renamed it as Helicobacter pylori. Helicobacter...
目的: 制备幽门螺杆菌(Helicobacter pylori)重组VacA的鸡蛋黄抗体IgY(VacA-IgY), 了解其理化特性和生物学活性, 评价其抗Helicobacter pylori感染的作用. 方法: 以纯化的重组VacA免疫产蛋鸡, 水稀释法提取蛋黄抗体(VacA-IgY), 硫酸铵沉淀法纯化IgY. 将一定浓度的VacA-IgY在不同温度的水浴中维持一定时间, 评价其耐热性; 在...
胃癌组织中幽门螺杆菌cagA和vacA的表达及与其感染的相关性
胃癌 幽门螺杆菌 细胞毒素相关基因A 空泡细胞毒素基因A
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2009/8/11
目的: 研究H pylori cagA和H pylori vacA在胃癌、胃黏膜不典型增生和胃炎组织中的表达及与H pylori感染的相关性. 方法: 采用Warthin-Starry嗜银染色法检测胃癌组织39例, 胃黏膜不典型增生组织24例和慢性胃炎组织33例中H pylori感染情况; PCR法检测上述标本中H pylori cagA和H pylori vacA的表达.
结果: 胃癌组织中H...
目的: 采用基因工程技术大量表达、提纯幽门螺杆菌(H pylori)细胞空泡毒素毒性片段, 和黏附素片段的融合蛋白, 以此为抗原, 制备高效价的抗VacA-HpaA蛋黄抗体(IgY). 方法: 大量培养重组菌pQE30-VacA-HpaA-DH5a, 诱导表达获得融合蛋白VacA-HpaA, Ni2+-NTA树脂纯化. 将纯化的重组蛋白免疫鸡, 水稀释法提取IgY, 硫酸铵沉淀法纯化浓缩VacA-...
靶向siRNA抑制幽门螺杆菌vacA表达
RNA干扰 空泡毒素A 幽门螺杆菌
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2009/8/6
目的: 观察靶向siRNA能否抑幽门螺杆菌(H pylori)vacA基因表达. 方法: 合成靶向H pylori vacA基因5对特异siRNA(实验组: vacA-s1, vacA-s2, vacA-s3, vacA-s4, vacA-s5)和1对非特异siRNA(对照组). 通过电穿孔法使siRNA转化至H pylor内, 观察转化效率和转化1, 6, 12, 24, 48 h mRNA和蛋...
幽门螺杆菌VacA重组蛋白对SGC7901胃癌细胞的作用
幽门螺杆菌 空泡毒素 重组蛋白 胃癌细胞
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2009/8/6
目的: 研究幽门螺杆菌空泡毒素(VacA)编码基因在大肠杆菌中的表达产物对SGC7901胃癌细胞的作用. 方法: 常规培养p E T 3 2 a - v a c A - E . c o l I BL21(DE3)工程菌株, IPTG诱导重组蛋白表达, 产物纯化后作用于胃癌细胞, 倒置显微镜及电镜等检测细胞生长情况. 结果: 重组蛋白作用于胃癌细胞后, 倒置显微镜下观察, 细胞生长受抑制, 其细胞空...
目的:通过对vacA重组工程菌表达产物包涵体的变性、复性,提高表达产物的生物学活性,建立ELISA方法,讨论VacA抗原规模生产的可行性.方法:利用已经构建的表达VacA抗原的工程菌,用IPTG诱导,表达的包涵体通过系列条件的变性、复性、透析,并经活性鉴定.所制抗原用以包被ELISA板,建立ELISA方法并确定抗原的生物学活性.结果:重组工程菌可表达Mr 3300的目的蛋白,表达形式为包涵体,条件...
幽门螺杆菌vacA毒性片段与hpaA融合基因的原核表达
幽门螺杆菌 vacA毒性片段 hpaA融合基因 原核表达
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2009/7/28
目的: 构建幽门螺杆菌vacA毒性片段(v)与hpaA融合基因的原核表达载体，并诱导表达，为制备具有治疗与预防作用的疫苗奠定基础.方法: 通过设计带有编码IL-3N端12个氨基酸引物，用PCR技术从pQE30-V质粒扩增出有接头的v基因，克隆至质粒pTrc99A-HpaA中与hpaA融合. 将融合基因插入原核表达载体pQE30，再将pQE30-V-HpaA转化大肠杆菌DH5 alpha，经IPTG...
噬菌体展示技术筛选幽门螺杆菌空泡毒素（VacA）基因结合蛋白
噬菌体展示 幽门螺杆菌 空泡毒素（VacA） 结合蛋白
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2008/6/11
噬菌体展示技术筛选幽门螺杆菌空泡毒素（VacA）基因结合蛋白。