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Squash preparation studies of germ cells in human fetal testes
testis preleptotene germ cells chromosomes
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2009/2/20
Testes from human fetuses 21 to 41 weeks of gestation were investigated using a modified squash technique. At all of the gestational ages examined, 90 to 95% of the germ cells were in interphase. Pr...
Analysis of human sperm function following exposure to the ionophore A23187. Comparison of normospermic and oligozoospermic men
A23187 sperm motility fertilization ohgozoospermia
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2009/2/20
Time exposure photomicrography and interspecific in vitro fertilization techniques have been used to compare the responses to the divalent cation ionophore A23187 of spermatozoa from normal fertile an...
Similar biochemical properties of human seminal plasma and epididymal alpha-1,4-glucosidase
1-4 glucosidase seminal plasma epididymis
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2009/2/20
We have studied some characteristics of alpha-1,4-glucosidases in human male reproductive organs in order to obtain information on the origin of the enzyme in seminal plasma. Acid and neutral enzyme...
Comparison of glycerol and a zwitter ion buffer system as cryoprotective media for human spermatozoa. Effect on motility, penetration of zona-free hamster oocytes, and acrosin/proacrosin
spermatozoa human cryopreservation
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2009/2/20
This study compared the cryoprotective effect of glycerol with that of a zwitter ion buffer system (TESTCY). Spermatozoa that are cryopreserved in the presence of glycerol possess a somewhat highe...
The binding patterns of antisera to sex steroids and human gonadotropins on human and rhesus monkey spermatozoa
human spermatozoa monkey spermatozoa hormone binding sites immunofluorescence steroids
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2009/2/20
The presence of different hormones on the surface of ejaculated spermatozoa was determined by immunofluorescence studies of the binding patterns of specific antisera to these hormones. There were st...
Human reproductive tissues contain thyrotropin releasing hormone（TRH）and TRH-homologous peptides
thyrotropin releasing hormone human prostate seminal vesicles testis epididymis
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2009/2/20
Thyrotropin releasing hormone (TRH) immunoreactive peptides occur in high concentration within rat ventral prostate and human semen. To extend these observations to human reproductive organs, tissue...
Human semen contains thyrotropin releasing hormone（TRH）, a TRH-homologous peptide, and TRH-binding substances
semen TRH vasectomy exclusion ion exchange high pressure chromatography
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2009/2/20
High levels of thyrotropin releasing hormone (TRH) and TRH-homologous peptide, with the general structure pGlu=X-Pro-NH2 where X is either Histidine (His) or a neutral amino acid residue, have been ...
Human sperm penetration in bovine cervical mucus. Clinical studies I
Human sperm penetration bovine cervical mucus
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2009/2/19
Penetration of spermatozoa in bovine cervical mucus (BCM) from two groups of men was investigated and correlated with the different parameters of spermograms from these same subjects. Sperm penetr...
Immunohistochemical study on the initiation of acid phosphatase secretion in the human prostate. Cytochemistry and biochemistry of acid phosphatases IV
Immunohistochemical study initiation acid phosphatase secretion human prostate
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2009/2/19
Acid phosphatase was purified from human prostatic tissue and from seminal plasma. Antisera to antigens from both sources were raised in rabbits. These antisera have been used for immunohistochemical ...
Sperm morphology assessment as an indicator of human fertilizing capacity
Sperm morphology assessment indicator human fertilizing capacity
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2009/2/19
Semen samples from 95 men were examined by routine semen analysis and specific histologic staining for sperm morphology. The men were classified into fertile and infertile groups on the basis of cli...
Relationship Between Fertilizing Ability and cAMP in Human Spermatozoa
capacitation of human spermatozoa cAMP in human spermatozoa fertilization of zona-free hamster eggs
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2009/2/19
The goal of this study was to investigate the relationship between cAMP and the fertilizing ability of human spermatozoa. Levels of cAMP were measured in human spermatozoa during 6-hour in vitro i...
Ultrastructural Observations on the Penetration of Human Sperm into the Zona Pellucida of the Human Egg In Vitro
ultrastructure zona pellucida human ova acrosome reaction
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2009/2/19
Sperm penetration into the zona of 46 human eggs was examined by electron microscopy. Preovulatory oocytes were aspirated at laparoscopy and inseminated by methods which produced normal pregnancie...
Human and Ram Seminal Plasma Both Contain a Calcium-Dependent Regulator Protein
Spermatozoa initiation of motility Ca2+-dependent regulator Ca2+-ATPase epididymis
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2009/2/19
Biochemical aspects of post-testicular sperm maturation have been studied. Partial purification of either human or ram seminal plasma yields a heat-stable, acidic protein which is a Ca2+-dependent...
A Method for Improvement of Sperm Quality of an Ejaculated Human Specimen Using Bovine Cervical Mucus as a Biological Filter
teratozoospermia bovine cervical mucus penetration test
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2009/2/19
A method is described for improving the quality of semen from subfertile men. Semen samples were filtered through bovine cervical mucus (collected at estrus) into spermatozoa-free seminal plasma (fr...
A Competitive In Vitro Assay of Human Sperm Fertilizing Ability Utilizing Contrasting Fluorescent Sperm Markers
Spermatozoa fertilization sperm motility
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2009/2/19
Fluorescein isothiocyanate (FITC) and tetramethyl rhodamine isothiocyanate (TRITC) were evaluated for use as contrasting fluorescent labels on living human spermatozoa. Unlabeled (control), FITC...