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中国林业科学研究院华北林业实验中心开发出精准性高的小型化Cas9基因编辑工具(图)
精准性高 小型化 Cas9基因 编辑工具
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2023/11/20
江苏省作物基因组学和分子育种重点实验室基因编辑团队在Plant Biotechnology Journal发布论文,系统评估了PAM松弛型CRISPR-Cas9植物基因组编辑工具的脱靶效应,提示ABE8e存在高比例脱靶
CRISPR-Cas9 基因编辑 水稻 脱靶效应
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2023/8/9
CRISPR-Cas9基因编辑工具凭借其高效、易用、灵活特性极大的改变了传统育种手段,研究人员为了扩大其靶向范围,设计了一系列SpCas9变体,包xCas9(识别5′-NG-3′、5′-GAA-3′、5′-GAT-3′ PAM)、SpCas9-NGv1和SpCas9-NG(识别5′-NG-3′ PAM)以及PAM-less SpRY然而,这些PAM-relaxed Cas9核酸酶是否会造成向导RN...
Off-Switch for CRISPR-Cas9 Gene Editing System Discovered
Off-Switch CRISPR-Cas9 Gene Editing System
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2017/1/9
UC San Francisco researchers have discovered a way to switch off the widely used CRISPR-Cas9 gene-editing system using newly identified anti-CRISPR proteins that are produced by bacterial vi...
CRISPR-Cas9 gene editing:check three times,cut once
CRISPR-Cas9 gene editing check three times cut once
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2015/12/7
Two new studies from UC Berkeley should give scientists who use CRISPR-Cas9 for genome engineering greater confidence that they won’t inadvertently edit the wrong DNA.The gene editing technique, creat...
Researchers Use DNA‘Clews’to Shuttle CRISPR-Cas9 Gene-Editing Tool into Cells
Researchers DNA Clews Shuttle CRISPR-Cas9 Gene-Editing Tool
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2015/9/8
CRISPR–Cas9 represents a promising platform for genome editing, yet means for its safe and efficient delivery remain to be fully realized. A novel vehicle that simultaneously delivers the Cas9 protein...