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Vitrification by Cryotop and the Maturation, Fertilization, and Developmental Rates of Mouse Oocytes
Vitrification Fertilization Maturation Cryotop Developmental Rate Oocyte
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2015/11/9
Background: Oocyte cryopreservation is an important part of modern fertility treatment. The effect of vitrification on the fertilization and developmental rates of embryo is still a matter of debate.
Ultrastructure of in vitro Matured Human Oocytes
In vitro Maturation Germinal Vesicle Oocytes Metaphase-I Oocytes Ultrastructure
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2015/10/9
Background: Approximately 20% of recovered oocytes are immature and discarded in intracytoplasmic sperm injection (ICSI) procedures. These oocytes represent a potential resource for both clinical and ...
Nitric oxide acts through different signaling pathways in maturation of cumulus cell-enclosed mouse oocytes
Nitric oxide Sildenafil Oocytes maturation Signaling pathway
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2009/12/16
Background: Nitric oxide (NO) have a dual action in mouse oocyte meiotic maturation which depends on its concentration, but the mechanisms by which it influences oocyte maturation has not been exactly...
Localization of tubulin and microtubules of in vivo fertilized rabbit oocytes
fertilization microtubules acrosome acrosin
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2009/2/20
The presence of tubulin throughout what appears to be sperm penetration tunnels of in vivo fertilized rabbit oocytes was demonstrated by both immunofluorescence and transmission electron microscopy,...
Comparison of glycerol and a zwitter ion buffer system as cryoprotective media for human spermatozoa. Effect on motility, penetration of zona-free hamster oocytes, and acrosin/proacrosin
spermatozoa human cryopreservation
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2009/2/20
This study compared the cryoprotective effect of glycerol with that of a zwitter ion buffer system (TESTCY). Spermatozoa that are cryopreserved in the presence of glycerol possess a somewhat highe...
Sperm motility enhancement by nitric oxide produced by the oocytes of fathead minnows, Pimephelas promelas
Fertilization CASA electron spin resonance oocyte
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2009/2/18
The effects of nitric oxide (NO) on sperm motility were examined in the fathead minnow, Pimephelas promelas, using computer-assisted sperm analysis (CASA). The observed effects underscore the dual n...
The use of two fluorescent dyes to identify sperm in a competitive binding assay to oocytes
fluorescent dyes identify sperm competitive binding assay oocytes
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2009/2/18
The relationship of most sperm laboratory assays to male fertility is inconsistent. Assays that measure traits required to fertilize oocytes are expected to have the most predictive value. A new ass...
Effect of bovine oviductal estrus-associated protein on the ability of sperm to capacitate and fertilize oocytes
Oviduct culture proteinsecretion oviductal fluid
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2009/2/12
At estrus, an oviduct-specific, estrus-associated glycoprotein (EAP) of molecular weight 85-95,000, is secreted by the oviductal epithelium and found in cannula-derived bovine oviductal fluid (ODF). T...
First Steps in the Development of a Functional Assay for Human Sperm Using Pig Oocytes
Sperm functionality assay species specificity sperm-oocyte interaction heterologous ICSI
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2008/12/24
The use of mammalian oocytes to assess human sperm functionality could be a helpful tool with potential applications in clinical and research programs. In an attempt to develop the pig model, the...
Does Seminal Plasma PSP-I/PSP-II Spermadhesin Modulate the Ability of Boar Spermatozoa to Penetrate Homologous Oocytes In Vitro?
Seminal plasma preservation sperm viability IVF pig
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2008/12/22
Low concentration (0.15 mg per million of spermatozoa) of seminal plasma–derived PSP-I/PSP-II spermadhesin heterodimer is able to preserve the viability of highly extended boar spermatozoa. Whether ...
Lignosulfonic Acid Blocks In Vitro Fertilization of Macaque Oocytes When Sperm Are Treated Either Before or After Capacitation
Contraception fucoidin microbicide polysulfonation zona pellucida
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2008/12/16
Lignin-derived macromolecules (LDMs) are biologically active compounds that affect a variety of cell-to-cell interactions including the inhibition of fertilization and embryo development in a number o...
EFFECT OF CYSTEAMINE ON THE RATE OF IN VITRO MATURATION OF OOCYTES IN TWO MEDIA
In vitro maturation of oocyte cysteamine glutathione mouse
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2010/2/8
Rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive techniques. In this study we investigated the effects of supplementation of cysteamine on the rate of in vitro ...