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Repeats as global DNA methylation marker in bovine preimplantation embryos
epigenetics retrotransposon bisulfite sequencing
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2017/2/21
DNA methylation undergoes dynamic changes and is a crucial part of the epigenetic regulation during mammalian early development. To determine the DNA methylation levels in bovine embryos, we applied a...
Siberian Sturgeon Oocyte Extract Induces Epigenetic Modifications of Porcine Somatic Cells and Improves Developmental Competence of SCNT Embryos
Epigenetic Modifications Somatic Cell Nuclear Transfer Siberian Sturgeon Oocyte Extract
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2016/5/16
Somatic cell nuclear transfer (SCNT) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. In the SCNT experiment, nuclear reprogramming is in...
筛选代表小鼠植入前胚胎紧密化相关基因的ESTIdentification of ESTs of Genes Related to Compaction in Preimplantation Embryos of Mouse
植入前 紧密化 抑制消减杂交 差异表达 小鼠
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2008/1/10
摘要
分别收集181及241枚昆明白小鼠8细胞早期胚胎及8细胞紧密化胚胎，采用SMART PCR方法直接合成胚胎双链cDNA。进而运用抑制消减杂交技术（SSH）对8细胞早期胚胎及8细胞紧密化胚胎的基因表达进行研究，并将所获得的差异表达产物按片段大小分段分离纯化后克隆入pUCm-T载体中，经PCR鉴定后挑选阳性克隆进行测序，筛选出27个代表8细胞早期胚胎和紧密化8细胞胚胎差别表达基因的cDNA片段...
Development of Bovine Nuclear Transfer Embryos Using Life-span Extended Donor Cells Transfected with Foreign Gene
Chromosomal Abnormality Foreign Gene Transfection Life-span Extended Donor Cell Somatic Cell Nuclear Transfer
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2016/4/22
This study was performed to determine the developmental potentials of nuclear transfer (NT) embryos using life-span extended cells transfected with a foreign gene as donor cells. A life-span extended ...
Effects of Melatonin on Gene Expression of IVM/IVF Porcine Embryos
Porcine Embryo RT-PCR Catalase Bax Caspase-3 Melatonin
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2016/4/18
The effect of melatonin on in vitro embryo development and the expression of antioxidant enzyme gene in preimplantation porcine embryos was determined by modified semi-quantitative single cell RT-PCR....