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Acidification of intracellular pH in bovine spermatozoa suppresses motility and extends viable life
Acidification of intracellular pH bovine spermatozoa motility viable life
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2008/12/25
Intracellular pH (pHi) was determined in ejaculated bovine spermatozoa using a ratiometric absorbance technique under various incubation conditions that drastically altered sperm motility. The pHi w...
Assessment of the acrosomal status of ram spermatozoa by RCA lectin-binding and partition in an aqueous two-phase system
acrosomal status ram spermatozoa RCA lectin-binding partition aqueous two-phase system
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2008/12/25
The acrosome reaction is an important marker for sperm function. Because different laboratory techniques may be used to detect this exocytotic process, the objective of this study was to investiga...
Progesterone-induced calcium influx in cynomolgus monkey（Macaca fascicularis）spermatozoa
Progesterone-induced calcium cynomolgus monkey spermatozoa
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2008/12/25
For in vitro capacitation to occur in cynomolgus monkey (Macaca fascicularis) spermatozoa, there is an absolute requirement for exogenous stimulation with the sperm activators, caffeine (1 mM) and d...
Cryopreservation of a small number of fresh human testicular spermatozoa and testicular spermatozoa cultured in vitro for 3 days in an empty zona pellucida
Cryopreservation human testicular spermatozoa testicular spermatozoa cultured vitro zona
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2008/12/25
It is known that the motility of human testicular sperm can be improved when they are cultured in vitro for a few days. The purpose of this study was to determine whether it is better to freeze huma...
Measurement of intracellular calcium concentration and plasma membrane potential in human spermatozoa using flow cytometry
Measurement intracellular calcium concentration plasma membrane potential human spermatozoa
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2008/12/25
We report 2 novel approaches using flow cytometry to measure intracellular calcium concentration and plasma membrane potential in human spermatozoa. Both approaches have the potential to measure dif...
Effects of accessory sex gland fluid on viability, capacitation, and the acrosome reaction of cauda epididymal bull spermatozoa
sex gland viability capacitation reaction bull spermatozoa
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2008/12/25
The effect of accessory sex gland fluid (AGF) on viability and acrosomal integrity of spermatozoa was examined with cauda epididymal spermatozoa and AGF from the same Holstein bull (n = 6). Surgical...
Maturation of epididymal spermatozoa in the nondomesticated guinea pigs Cavia aperea and Galea musteloides
Maturation epididymal spermatozoa nondomesticated guinea pigs Cavia aperea Galea musteloides
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2008/12/25
The physiological changes occurring in spermatozoa in the male reproductive tract of 2 nondomesticated species of South American guinea pigs with different mating systems were studied. Cavia apere...
DNA integrity in human spermatozoa：relationships with semen quality
DNA integrity human spermatozoa relationships semen quality
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2008/12/26
The literature contains conflicting evidence regarding the existence of DNA damage in spermatozoa from infertile male patients. To examine this phenomenon, we have studied ejaculated spermatozoa from ...
DNA integrity in human spermatozoa：relationships with semen quality
DNA integrity human spermatozoa relationships semen quality
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2008/12/25
The literature contains conflicting evidence regarding the existence of DNA damage in spermatozoa from infertile male patients. To examine this phenomenon, we have studied ejaculated spermatozoa fro...
Reproductive Failure in Patients With Various Percentages of Macronuclear Spermatozoa：High Level of Aneuploid and Polyploid Spermatozoa
Chromosomal abnormalities meiosis FISH nuclear surface
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2008/12/24
The aim of this study was to describe the association between various percentages of macronuclear spermatozoa (MNSs), sperm chromosomal abnormalities, and reproductive failure in 4 patients. One pat...
GM1 Dynamics as a Marker for Membrane Changes Associated With the Process of Capacitation in Murine and Bovine Spermatozoa
Rafts ganglioside cholesterol cholera toxin annulus
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2008/12/24
We previously showed that in live murine and bovine sperm heads, the ganglioside GM1 localizes to the sterol-rich plasma membrane overlying the acrosome (APM). Labeling GM1 using the pentameric c...
Impact of Ca2+ Flux Inhibitors on Acrosome Reaction of Hamster Spermatozoa
Ca2+ Inhibitors Acrosome Reaction Hamster Spermatozoa
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2008/12/24
Ca2+ plays a prominent role in the regulation of critical functions of spermatozoa, such as capacitation, acrosome reaction (AR), and fertilization. While there is consensus that Ca2+ is essential, ...
Abnormal Morphology of Spermatozoa in Cytochrome P450 17-hydroxylase/17, 20-lyase（CYP17）Deficient Mice
Gene deletion steroidogenesis mitochondria architecture fertility
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2008/12/24
Cytochrome P450 17-hydroxylase/17, 20-lyase (CYP17) is crucial for cortisol and sex steroid biosynthesis. In a previous study we examined CYP17 function by generating mice with a targeted CYP17 d...
The Response of Bovine Spermatozoa to Bicarbonate and Its Use to Assess the Influence of Added Oviductal Epithelial Proteins on Cryopreservation
Isthmus ampulla motility sperm-oviduct interactions
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2008/12/24
The oviduct is a crucial organ for fertilization and has been demonstrated to perform a variety of interactions with spermatozoa ranging from sperm storage, to stabilizing sperm membranes and red...
Characterizing the Glycocalyx of Poultry Spermatozoa：I. Identification and Distribution of Carbohydrate Residues Using Flow Cytometry and Epifluorescence Microscopy
Turkey chicken semen glycoconjugate lectin
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2008/12/24
The aim of the present work was to use a battery of lectins to 1) delineate the carbohydrate content of sperm glycocalyx in the turkey and chicken using flow cytometry analysis, and 2) evaluate the ...