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Comparison between human sperm preservation medium and TEST-yolk buffer on protecting chromatin and morphology integrity of human spermatozoa in fertile and subfertile men after freeze-thawing procedure
Acridine orange chromatin normality cryopreservation
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2008/12/29
The aim of this study was to identify the detrimental effect of the freeze-thaw process on chromatin integrity and morphology of human spermatozoa, and to determine whether human sperm preservation ...
Localization of clusterin on freeze-preserved bull spermatozoa before and after glass wool-sephadex filtration
Immunofluorescence abnormal spermatozoa semen
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2008/12/29
Clusterin is a major protein in bull reproductive tract secretions and sperm membrane extract. A polyclonal antibody was produced against clusterin from bull cauda epididymal fluid (CEF) and used fo...
The role of glucose in supporting motility and capacitation in human spermatozoa
Acrosome reaction ATP hamster oocyte penetration
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2008/12/29
Glucose has been reported to be beneficial to human sperm for optimal capacitation and fertilization, although it is unclear whether glucose is required for providing extra metabolic energy through gl...
Cryopreservation-Thawing of fractionated human spermatozoa is associated with membrane phosphatidylserine externalization and not DNA fragmentation
Phosphatidylserine translocation TUNEL annexin V
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2008/12/29
The objective of these studies was to evaluate the effect of cryopreservation-thawing of human spermatozoa on DNA fragmentation and membrane integrity. This was a prospective, controlled cohort stud...
A redox-regulated tyrosine phosphorylation cascade in rat spermatozoa
Epididymal maturation cAMP-dependent phosphorylation
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2008/12/29
Rat spermatozoa from both the caput and cauda epididymidis were shown to generate superoxide anion (O2-.) both spontaneously and following stimulation with NAD(P)H. Caput spermatozoa gave a signific...
Susceptibility of glycolytic enzyme activity and motility of spermatozoa from rat, mouse, and human to inhibition by proven and putative chlorinated antifertility compounds in vitro
glycolytic enzyme activity motility spermatozoa rat mouse human inhibition
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2008/12/26
Nonhormonal contraceptives that act by blocking energy metabolism within sperm have the advantage over spermatogenic inhibitors by their fast onset of infertility and their almost immediate restorat...
Accessory sperm as an indication of fertilizing ability of rabbit spermatozoa frozen in egg yolk-acetamide with detergent
Accessory sperm fertilizing ability of rabbit spermatozoa
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2008/12/26
Many factors besides initial semen quality affect fertilization rates as sperm interact with the environment of the female reproductive tract. One of these factors is sperm transport, which can be eva...
Morphology of testicular spermatozoa obtained by testicular sperm extraction in obstructive and nonobstructive azoospermic men and its relation to fertilization success in the in vitro fertilization-intracytoplasmic sperm injection system
Morphology testicular spermatozoa testicular sperm extraction azoospermic men
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2008/12/26
The aim of the present study was to evaluate the morphology of testicular spermatozoa by 3 different determinants. Sperm cells were obtained and their morphology was evaluated from 27 testicular spe...
Changes in motion characteristics, plasma membrane integrity, and acrosome morphology during cryopreservation of buffalo spermatozoa
plasma membrane integrity morphology cryopreservation of buffalo spermatozoa
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2008/12/26
Motion characteristics, plasma membrane integrity, and acrosome morphology of buffalo spermatozoa after different stages of cryopreservation (ie, dilution, cooling to 4 degrees C, equilibration at 4 d...
Lack of significant morphological differences between human X and Y spermatozoa and their precursor cells（spermatids）exposed to different prehybridization treatments
significant morphological huma X and Y spermatozoa precursor cells
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2008/12/26
Human X and Y spermatozoa were previously compared by several nonmolecular techniques. Recent studies show that in many of the previous investigations, the methods used to identify the spermatozoa wer...
Effects of methyl-beta-cyclodextrin on cryosurvival of boar spermatozoa
methyl-beta-cyclodextrin cryosurvival boar spermatozoa
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2008/12/26
Methyl-beta-cyclodextrin (MBCD), which leads to the stimulation of cholesterol efflux from the cell membrane, was examined for its ability to increase the cryosurvival of spermatozoa from Gottingen mi...
Actin and actin-binding proteins in bovine spermatozoa：potential role in membrane remodeling and intracellular signaling during epididymal maturation and the acrosome reaction
proteins bovine spermatozoa potential role signaling epididymal maturation
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2008/12/26
The actin cytoskeleton influences a wide range of functions in nonmuscle somatic cells, including shape, movement, and interactions with extracellular matrices. The role of actin in mammalian male ger...
Separation of ram spermatozoa bearing X and Y chromosome by centrifugal countercurrent distribution in an aqueous two-phase system
ram spermatozoa X and Y chromosome centrifugal countercurrent aqueous two-phase system
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2008/12/25
The availability of reliable quantification techniques of X and Y chromosome-bearing spermatozoa in a given insemination dose would allow further approaches to their separation, which is a topic o...
Correlation between clusterin-positive spermatozoa determined by flow cytometry in bull semen and fertility
Correlation clusterin-positive spermatozoa cytometry bull semen fertility
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2008/12/25
The objectives were to 1) develop a rapid and accurate method for detection of clusterin-positive spermatozoa (CPS) in bull semen and 2) determine the utility of incidence of CPS for prediction of f...
Calpain and calpastatin are located between the plasma membrane and outer acrosomal membrane of cynomolgus macaque spermatozoa
Calpain calpastatin plasma membrane outer acrosomal membrane cynomolgus macaque spermatozoa
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2008/12/25
Mammalian sperm must undergo an acrosome reaction prior to penetration of the zona pellucida and subsequent fusion with an oocyte. Sperm gain the capability to acrosome react after a period of capac...