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Experimental cryptorchidism in the adult mouse：II. A hormonal study
Leydig cells FSH LH testosterone hCG radioimmunoassay
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2009/2/6
Serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels and secretory capacity of the in vitro stimulated testis were determined in control and bilateral cryptorc...
Experimental cryptorchidism in the adult mouse：I. Qualitative and quantitative light microscopic morphology
Testis Leydig cells stereology hyperplasia,hypotrophy
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2009/2/6
Morphologic changes in the testes of adult mice after experimentally induced cryptorchidism were studied by light microscopy and stereology. Increasing duration of cryptorchidism resulted in a gradu...
Tissue and species specificity of mouse ductus deferens protein
ductus deferens major protein monoclonal antibodies androgens mouse
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2009/2/6
The ductus deferens of the mouse contains a major protein with a molecular weight of 34.5 kd called mouse vas deferens protein (MVDP). Immunofluorescence histochemistry and immunoblotting with monoclo...
Evidence for a role of cysteinyl leukotrienes in mouse and human sperm function
spermatozoa fertilization leukotrienes receptor antagonist FPL-55712
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2009/1/5
The in vitro fertilizing capacity of mouse spermatozoa was inhibited by FPL-55712, a receptor antagonist of the cysteinyl-containing leukotrienes LTC4, LTD4, and LTE4. Inhibition occurred whether ...
Cordyceps sinensis and its fractions stimulate MA-10 mouse Leydig tumor cell steroidogenesis
Human chorionic gonadotropin steroidogenic acute
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2008/12/29
The effects of Cordyceps sinensis (CS) and its extracted fractions on steroidogenesis in MA-10 cells were determined. Different concentrations of CS and 3 fractions of CS (F1, a water-soluble polysa...
Immunomagnetic isolation and long-term culture of mouse type A spermatogonia
Germ cells c-kit Ep-CAM magnetic beads Sertoli
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2008/12/29
In the mammalian testis, type A spermatogonia proliferate and differentiate into sperm under the tight control of both endocrine and paracrine factors. In order to study the complex process of sperm...
Mouse seminal vesicle secretory protein of 99 amino acids（MSVSP99）：characterization and hormonal and developmental regulation
Mouse seminal vesicle secretory protein developmental regulation
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2008/12/29
Polyclonal antibodies have been generated to investigate the localization, tissue and species distribution, androgen regulation, and ontogeny of a protein secreted by mouse seminal vesicle, designat...
Susceptibility of glycolytic enzyme activity and motility of spermatozoa from rat, mouse, and human to inhibition by proven and putative chlorinated antifertility compounds in vitro
glycolytic enzyme activity motility spermatozoa rat mouse human inhibition
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2008/12/26
Nonhormonal contraceptives that act by blocking energy metabolism within sperm have the advantage over spermatogenic inhibitors by their fast onset of infertility and their almost immediate restorat...
The effect of cardiac arrest on the blood-testis barrier to albumin, tumor necrosis factor-alpha, pituitary adenylate cyclase activating polypeptide, sucrose, and verapamil in the mouse
blood-testis albumin tumor necrosis factor-alpha pituitary adenylate cyclase
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2008/12/26
Impotence commonly occurs after events such as acute myocardial infarction, coronary bypass, head trauma, and cerebral bleeding, including subarachnoid hemorrhage. We hypothesize that the hypoxia ac...
Expression of crisp-1 mRNA splice variants in the rat epididymis, and comparative analysis of the rat and mouse crisp-1 gene regulatory regions
crisp-1 mRNA rat epididymis rat and mouse crisp-1 gene
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2008/12/26
The rat Crisp-1 gene encodes Protein DE (acidic epididymal glycoprotein; AEG), a glycoprotein secreted by the epididymal epithelium that associates with maturing sperm and has been implicated in the p...
Detection of the mouse acrosome reaction by acid phosphatase Comparison with chlortetracycline and electron microscopy
mouse acid phosphatase chlortetracycline electron microscopy
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2008/12/26
The sperm acrosome is a uniquely regulated secretory vesicle containing several hydrolase enzymes, including acid phosphatase (AP). The exocytotic event that releases these enzymes, the acrosome react...
Mouse Spam1（PH-20）：evidence for its expression in the epididymis and for a new category of spermatogenic-expressed genes
Mouse Spam epididymis spermatogenic-expressed genes
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2008/12/25
The gene for the sperm adhesion molecule 1 (PH-20), SPAM1, has been known to be testis-specific and exclusively haploid expressed. We show that in mice, the 2 common isoforms of the protein (Spam1...
Targeted disruption of the mouse prosaposin gene affects the development of the prostate gland and other male reproductive organs
mouse prosaposin gene prostate gland male reproductive organs
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2008/12/25
The prosaposin gene encodes a 65-70 kilodalton (kd) protein, which is secreted or targeted to lysosomes. In lysosomes, prosaposin is the precursor of 4 activator proteins, designated saposins A, B...
Acquisition of androgen-mediated expression of mouse vas deferens protein（MVDP）gene in cultured epithelial cells and in vas deferens during postnatal development
androgen-mediated mouse vas deferens gene cultured epithelial cells
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2008/12/25
We used cultured vas deferens epithelial cells (VDECs) as a model system to determine the conditions that allow mouse vas deferens protein (MVDP) gene expression and acquisition of androgen responsi...
Immunolocalization of CA II and H+ V-ATPase in epithelial cells of the mouse and rat epididymis
Immunolocalization CA II H+ V-ATPase epithelial cells mouse rat epididymis
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2008/12/25
Acidification of the epididymal lumen has been suggested to play an important role in sperm functions; however, the cell types, pumps, and mechanisms involved have not been fully addressed. In thi...