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Antioxidant potential of human serum albumin：role in the recovery of high quality human spermatozoa for assisted reproductive technology
Percoll Cypridina luciferin analog MCLA superoxide
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2009/2/17
Human serum albumin (HSA) is being considered as an alternate media for sperm enrichment in assisted reproductive technology (ART) because of recent concern with the use of Percoll. In this study,...
Influence of centrifugation regimes on motility, yield, and cell associations of mouse spermatozoa
Cryopreservation mechanical sensitivity damage recovery
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2009/2/17
Mouse sperm are exceptionally sensitive to mechanical forces associated with pipetting and mixing. This characteristic raised the question of the sensitivity of mouse sperm to centrifugation, a st...
The effects of antifreeze peptide III (AFP) and insulin transferrin selenium（ITS）on cryopreservation of chimpanzee（Pan troglodytes）spermatozoa
Semen acrosome CAMA FITC-PSA
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2009/2/17
We investigated the effects of antifreeze peptides (AFP) and insulin transferrin selenium (ITS) on the motility and membrane integrity of chimpanzee (Pan troglodytes) spermatozoa after chilling (0...
Cytoplasmic extrusion and the switch from creatine kinase B to M isoform are completed by the commencement of epididymal transport in human and stallion spermatozoa
Maturity and function men stallions fertility cytoplasmic
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2009/2/17
Although in several species there is a relationship between epididymal sperm transport and fertility, in human in vitro fertilization (IVF), spermatozoa recovered from the caput epididymidis or ev...
Enhanced susceptibility of follicle-stimulating-hormone-deprived infertile bonnet monkey（Macaca radiata）spermatozoa to dithiothreitol-induced DNA decondensation in situ
Enhanced susceptibility follicle-stimulating-hormone-deprived infertile bonnet monkey dithiothreitol-induced DNA decondensation
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2009/2/17
Immunoneutralization of endogenous follicle-stimulating hormone (FSH) of adult male monkeys leads to oligospermia and infertility despite unchanged testosterone levels. The inability of these monkey...
Premature capacitation of bovine spermatozoa is initiated by cryopreservation
Premature capacitation bovine spermatozoa cryopreservation
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2009/2/17
Poor motility and abnormal acrosomal morphology only partially explain the reduced fertility of cryopreserved bovine spermatozoa. To test the hypothesis that cryopreservation procedures (dilution,...
PH-20 is a sperm plasma-membrane protein that has been shown to have hyaluronidase activity in several mammalian species including nonhuman primates. In this investigation, the PH-20 protein was c...
Continuous assessment of human spermatozoa viability during cryopreservation
Sperm cryomicroscopy fluorescent viability probes
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2009/2/17
Cryomicroscopy has enabled direct observation of freezing and thawing of human spermatozoa. When used with a fluorescent viability kit, sperm membrane damage was not apparent down to temperatures of...
Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa
FITC-PNA hemizona acrosome reaction
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2009/2/17
Peanut agglutinin (PNA) was used to assess the sperm acrosomal status and the acrosome reaction during gamete interaction in the equine species. PNA exclusively binds to the outer acrosomal membra...
Snow leopard（Panthera uncia）spermatozoa are sensitive to alkaline pH, but motility in vitro is not influenced by protein or energy supplements
Felid comparative biology sperm culture medium sperm function capacitation
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2009/2/16
To better understand the biology of snow leopard spermatozoa and to facilitate developing assisted reproduction, a series of studies was conducted to: 1) identify the component(s) of complex cultu...
Maturation of monkey spermatozoa in the epididymis with respect to their ability to undergo the acrosome reaction
Capacitation primate development epididymis macaque
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2009/2/16
Spermatozoa obtained along the length of the epididymides from five macaque monkeys (Macaca fascicularis) were examined for their ability to undergo the acrosome reaction under basal conditions and ...
Cryopreservation reduces the ability of equine spermatozoa to attach to oviductal epithelial cells and zonae pellucidae in vitro
equine spermatozoa oviductal epithelial cells zonae pellucidae
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2009/2/13
Two bioassays were used to evaluate the interaction of fresh and cryopreserved equine semen with oviductal epithelial cells (OEC) and with the zona pellucida (ZP). Split ejaculates were either store...
Glycosidic specificity of fucosyltransferases present in rat epididymal spermatozoa
Glycosidic specificity fucosyltransferases present rat epididymal spermatozoa
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2009/2/13
We have recently demonstrated multiple fucosyltransferase (FT) activity in rat spermatogenic cells. To complement these findings, here we identify and partially characterize the glycosidic linkage s...
Cryopreservation extenders affect calcium flux in bovine spermatozoa during a temperature challenge
Milk egg yolk-Tris indo-1 acetoxymethyl ester cold
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2009/2/13
Bovine spermatozoa are commercially cryopreserved by diluting the cells in media, known as extenders, followed by slow cooling and freezing. Previous work has shown that this process of cryopreser...
Acrosin activity of human spermatozoa by means of a simple gelatinolytic technique：a method useful for IVF
Human spermatozoa acrosin activity male infertility
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2009/2/13
Acrosin activity was determined using a gelatinolysis technique in 100-microliter semen aliquots of 114 patients (normozoospermia, n = 90; asthenozoospermia, n = 12; oligozoospermia, n = 10; polyz...