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The dose- and time-dependent responses for the interstitial and tubular compartments in irradiated adult rat testes are described. Leydig cell dysfunction, as indicated by increased serum LH (to a m...
Sixty prepubertal rats were subjected to unilateral spermatic cord torsion for a duration of 0, 1, 3, 5, 9 or 12 hours. At the end of this period, the ipsilateral testes either were removed for imme...
Male rats exposed in utero to delta 9-tetrahydrocannabinol (delta 9-THC) had lower levels of testosterone (T) and luteinizing hormone (LH) prior to puberty (P less than 0.01). At puberty, the levels...
Hypophysectomized male rats were administered LH or LH + FSH for 14 days and subjected to in vivo micropuncture collection of reproductive tract fluids to determine if FSH alters the compartmentaliz...
Cellular and media concentrations of polyamines in Sertoli cell cultures were determined by fluorescent spectroscopy of dansylated compounds after separation by high-performance liquid chromatograph...
Rat seminiferous tubule segments have been cultured in chemically defined medium (F12/DMEM 1:1) without added hormones or growth factors. The segments (1-2 mm) were isolated from defined stages of t...
The stages of the rat seminiferous epithelial cycle have been isolated for flow cytometric analysis of DNA and for culture, using transillumination-assisted microdissection. Precise stages have been...
Immobilin, the highly viscoelastic glycoprotein isolated from rat cauda epididymal fluid, exhibits all of the key biochemical characteristics of a mucin: 1) it has a very high molecular weight (will...
The absorption of L-carnitine from the duct of the proximal regions of the rat epididymis was investigated using a stopped-flow, split-droplet microperfusion technique. L-carnitine was absorbed from...
The isolation and culture of ciliated and nonciliated cells from rat ductuli efferentes is described. Fragments of epithelium obtained after two collagenase digestions attached to plastic and to ext...
8-Azido cAMP photoaffinity labeling of cAMP-dependent protein kinase regulatory subunits (R1 = 49 K;R2 = 55K) was done on spermatozoa from species lacking, and species containing an epididymis. Sper...
The activity of N-acetyl-beta-D-glucosaminidase (NAG, 60.1 units/mg protein) and of acid phosphatase (57.7 units/mg protein) in fluid from the cauda epididymidis formed without any contribution from...
The endocytic activity of Leydig cells was examined by electron microscopy following the injection, into the interstitial space, of tracers used to examine fluid-phase endocytosis, ie, native ferrit...
The distribution of cellular retinol-binding protein (CRBP) and cellular retinoic acid-binding protein (CRABP) in rat testis and epididymis was examined by the peroxidase-antiperoxidase immunolocali...
The effects of hyperprolactinemia on testicular structure and pituitary-gonadal function were investigated in male rats. Hyperprolactinemia was induced in the Wistar-Furth rat by implantation of t...

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