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Discriminant analysis indicates a single sperm protein(SP22)is predictive of fertility following exposure to epididymal toxicants
Epididymis biomarker
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2009/2/17
In a previous study, we found that ethane dimethanesulphonate (EDS) compromised the fertilizing ability of proximal cauda epididymal sperm from the rat within 4 days of exposure, an effect that pers...
Mouse epididymal sperm contain active P450 aromatase which decreases as sperm traverse the epididymis
Spermatozoa estrogen efferent ductules cytoplasmic droplet testicular excurrent ducts
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2009/2/16
Recently we reported that mouse germ cells in the testis contain active P450 aromatase (P450arom), the enzyme that converts androgens to estrogens. This finding suggested that germ cells have the ...
Regulation of the epididymal synthesis of P26h, a hamster sperm protein
Androgens epididymis testosterone sperm surface protein
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2009/2/16
We have previously identified a 26-kDa epididymal hamster sperm glycoprotein (P26h) that is involved in gamete interaction. This protein is added to the acrosomal cap during epididymal transit of sp...
Glycosidic specificity of fucosyltransferases present in rat epididymal spermatozoa
Glycosidic specificity fucosyltransferases present rat epididymal spermatozoa
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2009/2/13
We have recently demonstrated multiple fucosyltransferase (FT) activity in rat spermatogenic cells. To complement these findings, here we identify and partially characterize the glycosidic linkage s...
The ethane dimethanesulfonate-induced decrease in the fertilizing ability of cauda epididymal sperm is independent of the testis
Epididymis sperm maturation in utero insemination fertilization toxicant
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2009/2/12
Several decades ago it was reported that when adult male rats were exposed to a single injection of 50 mg/kg body weight ethane dimethanesulfonate (EDS) and mated with untreated females, average l...
Stimulation of cryopreserved epididymal spermatozoa of the domestic cat using the motility stimulants caffeine, pentoxifylline, and 2'-deoxyadenosine
Domestic cat motility stimulants hyperactivation
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2009/2/12
We have investigated the effects of caffeine, pentoxifylline, and 2'-deoxyadenosine on the motion characteristics and longevity of domestic cat spermatozoa. Freshly collected or cryopreserved dome...
Computer-assisted semen analysis(CASA)of epididymal sperm from the domestic cat
Computer-assisted semen analysis domestic cat normozoospermia teratozoospermia sperm morphology
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2009/2/11
Motion characteristics of epididymal sperm from domestic cats exhibiting a high (> 60%; normozoospermic; n = 21) or low (< 40%; teratozoospermic; n = 6) occurrence of structurally normal spermatozoa...
Testicular regulation of epididymal protein secretion
Testicular regulation epididymal protein secretion
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2009/2/10
The effects of different androgen and testicular fluid levels on the protein synthesis and secretion of the epididymis of mice and rats were examined. In mice, the in vitro protein synthesis and s...
Biochemical alterations in the proacrosin-acrosin system during epididymal maturation of the rat spermatozoa
Biochemical alterations proacrosin-acrosin system epididymal maturation rat spermatozoa
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2009/2/10
Acrosin, an acrosomal serine protease, is believed to have a role in fertilization. The enzyme is synthesized in an enzymatically inactive precursor form, proacrosin, and is processed to enzymatical...
Correlation between changes in rat sperm membrane lipids, protein, and the membrane physical state during epididymal maturation
Electron paramagnetic resonance spectroscopy(EPR) spin label 5-doxyldecane (5-Nb)
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2009/2/7
An enriched plasma membrane fraction was isolated from caput, corpus, and cauda rat spermatozoa and analyzed for lipid and protein content, thermal phase transition temperature using electron para...
The testicular and epididymal luminal amino acid microenvironment in the rat
Microperfusion glutamate osmolality absorption secretion
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2009/2/6
Concentrations of amino acids were measured in arterial and testicular venous blood, and in fluids from the seminiferous tubule, rete testis, and the caput, corpus, and cauda epididymidis. There wer...
Seminal alpha-glucosidase activity as a marker of epididymal pathology in nonazoospermic men consulting for infertility
human epididymis a-glucosidase epididymal markers clinical epididymal abnormalities functional epididymal failure
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2009/2/6
Glucosidase (alpha G) activity was measured in sperm free seminal plasma from 1200 patients consulting for primary infertility, in whom clinical examination of epididymides revealed some abnormaliti...
Leptin secretion from the epididymal fat pad is increased by the sexual maturation of the male rat
epididymal fat pad sexual maturation male rat
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2008/12/26
FloridaAlthough leptin has been implicated as an important factor in triggering the onset of puberty in females, much less is known about the role of this adipose tissue hormone in the sexual maturati...
Epididymal growth and differentiation are altered in human cryptorchidism
Epididymal growth differentiation human cryptorchidism
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2008/12/26
Despite the knowledge and histological classification of testicular lesions, epididymal lesions associated with cryptorchidism are not well defined and only macroscopic alterations have been reporte...
Actin and actin-binding proteins in bovine spermatozoa:potential role in membrane remodeling and intracellular signaling during epididymal maturation and the acrosome reaction
proteins bovine spermatozoa potential role signaling epididymal maturation
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2008/12/26
The actin cytoskeleton influences a wide range of functions in nonmuscle somatic cells, including shape, movement, and interactions with extracellular matrices. The role of actin in mammalian male ger...