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It has been known that glutamine synthetase (GS, L-glutamate ammonia ligase) is an enzyme that catalyzes ATP-dependent condensation of glutamate and ammonia to form glutamine, and that GS and growth ...
This is the first successful report on producing the endangered domestic fowl, Kureko Dori (KD), which is a Kumamoto prefectural natural treasure, using germline chimeras transplanted from primordial ...
Effect of feeding astaxanthin (Ax) provided as Phaffia rhodozyma on T cell population and responsiveness to mitogen, concanavalin A (Con A) or phytohemaggulutinin (PHA), of splenic mononuclear cells (...
The present study was carried out to develop techniques for introducing exogenous DNA into primordial germ cells (PGCs) and expressing the introduced DNA efficiently in the gonads of developing chicke...
Primordial germ cells (PGCs) of white leghorn Maria line hens embryos as a donor were transferred white leghorn Laura line hens embryos as a recipient. During stage 12 to 15 of incubating fertilized ...
The cells in the center of the area pellucida of the blastoderm have been used as donors for production of chimeric chickens, but the developmental fate of the cells has not been analyzed in detail. A...
Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the large size of their yolk structure. Cryopreservation of primordial...
Interspecies or even inter-genus somatic nuclear transfer is considered to be an effective means for conserving a wide variety of avian genetic resources. However, somatic nuclear transfer offspring p...
The aims of this study was to find out the histological differences of the mucosa and comparative analysis of Ig-containing plasma cells among the different segments of the gastrointestinal tract of b...
We cryopreserved gonadal germ cells (GGCs) collected from 7-day-old chick embryos using the vitrification method. Gonadal germ cells were suspended in vitrification medium and plunged directly into li...
A variety of fruits have traditionally been used in Asian cultures to enhance resistance to diseases and treat cancers. However, limited information exists on the underlying mechanisms responsible fo...
The generation of germline chimeras by the transfer of primordial germ cells (PGCs) requires incorporation of the PGCs of the donor into the gonadal tissue of the recipient embryo. We investigated the...
The current study aimed to determine the optimal time of busulfan (1,4-butanediol dimethanesulfonate) administration for the preparation of suitable recipient embryos to generate germline chimeras in ...
A novel strategy has been developed to generate muscular dystrophy chickens by means of germline chimeras. Donor embryos were obtained from the New Hampshire chicken; NH-413 strain which have genes re...
Chicken primordial germ cells (PGCs) circulate in the bloodstream and migrate to germinal ridges.In order to assess the migration frequencies of PGCs to left or right germinal ridges, PGCs were transf...

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