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microRNA155在长白猪和通城猪骨骼肌发育过程中的表达分析
猪 miR155 骨骼肌 发育
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2013/12/2
本研究旨在分析miR155在长白猪（瘦肉型）和通城猪（脂肪型）骨骼肌发育过程中的表达变化，以了解其在猪肌肉发育中的重要作用。选用长白猪和通城猪不同发育时期(胚胎期12个时期，出生后10个时期)骨骼肌为材料，利用StemLoop RTPCR方法检测miR155的表达变化。此外，本研究还分析了miR155在成年通城猪各组织的表达。结果，miR155在长白和通城猪骨骼肌发育过程中差异表达呈现...
本研究旨在探索流式细胞仪分离精子在猪性别控制中的应用。使用流式细胞仪分离猪XY精子,而后通过母猪输卵管授精生产“预知”性别的仔猪，并使用吖啶橙染色法检测粗分离对精子核酸含量的影响。结果，成功利用分离获得的猪X和Y精子对母猪进行输卵管授精，母猪怀孕率、产仔率均为100％；输Y精子母猪产仔雄性率100％（♂6/6），对照母猪产仔雄性率57.14％（♂8/14）；3头输X精子母猪产母仔率91.67％（♀...
苹果酸酶在葡萄糖和胰岛素诱导鹅肝脂肪变性中的作用研究
鹅 苹果酸酶 葡萄糖 胰岛素
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2013/12/2
本研究旨在研究不同浓度葡萄糖和胰岛素诱导鹅肝脂肪变性过程中苹果酸酶（Malic enzyme,ME）的活性及其mRNA表达情况，以探讨ME在鹅肝脂肪变性中的作用。根据鸡ME基因序列保守区设计引物，通过RTPCR、克隆测序等技术扩增鹅ME基因；分离、培养四川白鹅原代肝细胞，添加不同浓度葡萄糖和胰岛素诱导肝细胞脂肪变性，检测细胞内甘油三酯（TG）含量、ME活性及其mRNA表达情况。结果发现，鹅ME基...
Methane and Nitrous Oxide Emissions from Livestock Agriculture in 16 Local Administrative Districts of Korea
Greenhouse Gas Methane Nitrous Oxide Carbon Dioxide Equivalent Emission Climate Change
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2016/5/11
This study was conducted to evaluate methane (CH4) and nitrous oxide (N2O) emissions from livestock agriculture in 16 local administrative districts of Korea from 1990 to 2030. National Inventory Repo...
Effects of Synchronization of Carbohydrate and Protein Supply in Total Mixed Ration with Korean Rice Wine Residue on Ruminal Fermentation, Nitrogen Metabolism and Microbial Protein Synthesis in Holstein Steers
By-product Dairy Steer Microbial Protein Synthesis Purine Derivative
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2016/5/10
Three Holstein steers in the growing phase, each with a ruminal cannula, were used to test the hypothesis that the synchronization of the hourly rate of carbohydrate and nitrogen (N) released in the r...
Transfer of Orally Administered Terpenes in Goat Milk and Cheese
Feed Tracers Terpenes Goat Blood Milk Cheese
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2016/5/10
The objective of the present study was to investigate the relationships between terpenes’ intake and their presence in animal tissues (blood and milk) as well as in the final product (cheese). Eight d...
Comparison of Growth Performance of Antibiotic-free Yorkshire Crossbreds Sired by Berkshire, Large Black, and Tamworth Breeds Raised in Hoop Structures
Berkshire Large Black Tamworth Antibiotic-free Yorkshire Crossbred Growth
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2016/5/10
The objective of this study was to compare body weight, ADG, and feed:gain ratio of antibiotic-free pigs from Yorkshire dams and sired by Yorkshire (YY), Berkshire (BY), Large Black (LBY) or Tamworth ...
Effects of Probiotic and Prebiotic on Average Daily Gain, Fecal Shedding of Escherichia Coli, and Immune System Status in Newborn Female Calves
Holstein Calves Multi-strain Probiotic Mannanoligosaccharides Beta-glucan E. coli Count
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2016/5/10
Thirty two Holstein female calves (initial body weight = 40±3.0 kg) were used to investigate the effects of probiotic and prebiotic on average daily gain (ADG), fecal E. coli count, white blood cell c...
Comparative Analysis on Antioxidative Ability of Muscle between Laiwu Pig and Large White
Pig Superoxide Dismutase Catalase Glutathione Peroxidase Malondialdehyde
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2016/5/10
This study was conducted to evaluate effects of storage temperatures (4°C and 20°C) and pig breeds (Laiwu pig and Large White pig) on the main antioxidative enzymes (superoxide dismutase, catalase, an...
Performance of Nursing Awassi Ewes Fed Different Levels of Bread By-product
Awassi Ewes Bread by Product Nutrient Intake Nutrient Digestibility Milk Production
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2016/5/10
Objective of this experiment was to evaluate the effect of partial substitution of barley grain with bread by-product (BB) on performance of Awassi ewes and their lambs. Forty Awassi ewes rearing sing...
Effect of Sodium Nitrate and Nitrate Reducing Bacteria on In vitro Methane Production and Fermentation with Buffalo Rumen Liquor
In vitro Gas Production Methane Nitrite Nitrate Reducing Bacteria Buffalo
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2016/5/10
Nitrate can serve as a terminal electron acceptor in place of carbon dioxide and inhibit methane emission in the rumen and nitrate reducing bacteria might help enhance the reduction of nitrate/nitrite...
Effect of Xylanase on Performance and Apparent Metabolisable Energy in Starter Broilers Fed Diets Containing One Maize Variety Harvested in Different Regions of China
Broiler Digestibility Maize Xylanase
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2016/5/9
The objective of this study was to investigate the variability in broiler performance, apparent metabolisable energy (AME) and ileal digestible energy (IDE) between five different maize samples fed wi...
Evaluation of Milk Trace Elements, Lactate Dehydrogenase, Alkaline Phosphatase and Aspartate Aminotransferase Activity of Subclinical Mastitis as and Indicator of Subclinical Mastitis in Riverine Buffalo (Bubalus bubalis)
Riverine Buffalo Somatic Cell Count Subclinical Mastitis Indicator Trace Minerals Enzyme Activity
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2016/5/9
Mastitis is a highly morbid disease that requires detection at the subclinical stage. Tropical countries like India mainly depend on milch buffaloes for milk. The present study was conducted to invest...
Effects of Boron Supplementation to Diets Deficient in Calcium and Phosphorus on Performance with Some Serum, Bone and Fecal Characteristics of Broiler Chickens
Boron Broiler Growth Metabolic Profile Bone Structure Fecal Excretion
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2016/5/9
Three levels of boron (0, 30, 60 ppm) were supplemented in practical corn-soybean based starter and grower diets, containing either adequate or inadequate Ca or P. A total of 1,800, 1-day-old sexed br...
Seasonal Changes in Serum Testosterone, LDH Concentration and Semen Characteristics in Markhoz Goats
Markhoz Goat, Semen Characteristics, Testosterone, Lactate Dehydrogenase
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2016/5/9
This experiment was conducted to study variations of serum testosterone and seminal characteristics of Markhoz male goats. Blood samples were obtained via jugular vein, and semen was collected by usin...