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钝裂银莲花花色素合成相关基因qRT-PCR内参基因的筛选
钝裂银莲花 实时荧光定量PCR 内参基因 筛选 稳定性 类黄酮/花青素合成途径
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2021/3/12
为了筛选适合于钝裂银莲花类黄酮/花青素合成途径中相关基因qRT-PCR表达分析时的内参基因，根据钝裂银莲花蓝/白不同花色花器官组织的转录组测序结果，选取了多聚泛素酶基因（polyubiquitin，UBQ）、微管蛋白基因（β-tubulin，β-TUB）、水通道蛋白基因（aquaporin，AQP）、肌动蛋白基因（actin，ACT）、甘油醛–3–磷酸–脱氢酶基因（glyceraldehyde-3...
为筛选茄子（Solanum melongena L.）高温胁迫下稳定表达的内参基因，以不同茄子品系（种）为研究对象，PCR技术对来自茄子高温胁迫转录组数据库8个候选内参基因（SmEF1a、SmEF2、Sm40sRPS29、Sm60sRPL24、SmTRX、SmCK I、SmDAHPS I、SmUCP）和SmActin在不同试验情况下进行表达检测，并结合GeNorm、NormFinder、BestK...
Evaluation of the effects of tropical tanniferous plants on rumen microbiota using qRT PCR and DGGE analysis
Sheep condensed tannin gas production methanogenic Fibrobacter succinogenes electrophoresis molecular techniques
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2015/6/24
Tanniferous forages may have bacteriostatic and/or bactericidal effect on different rumen microbial populations. We investigated the influence of the tropical tanniferous plants Styzolobium aterrimum ...
Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR
avian influenza virus qRT-PCR TagMan probe
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2015/4/1
This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) between January and April 2006 in the Czech...