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Comparison of growth, development, survivorship and food utilization of two color varieties of Tenebrio molitor
Tenebrio molitor color variety growth rate survival rate developmental duration food utilization efficiency
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2011/12/28
Tenebrio molitor L. is an important resource insect. Based on the cuticular color of 60-day-old larvae, the yellow- and black-color varieties of T. molitor were obtained through natural selection of ...
摘要利用反转录-多聚酶链式反应(RT-PCR)的方法, 克隆黄粉甲虫(Tenebrio molitor)抗冻蛋白基因cDNA片段并进行序列分析和原核表达。同源性分析表明, 获得9条新cDNA片段, 与黄粉甲虫抗冻蛋白基因家族的其他基因序列具有较高的同源性。重组质粒pGEX-4T-1-tmafp-XJ430, 转化E.coli BL21进行原核表达, SDS-PAGE分析结果表明, 抗冻蛋白基因以可...