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Effect of Antioxidant Flavonoids (Quercetin and Taxifolin) on In vitro Maturation of Porcine Oocytes
Porcine Oocyte Antioxidants Quercetin Reactive Oxygen Species [ROS]
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2016/5/19
Quercetin (QT) and taxifolin (TF) are structurally similar plant-derived flavonoids that have antioxidant properties and act as free radical scavengers. The objective of this study was to investigate ...
Vitrification by Cryotop and the Maturation, Fertilization, and Developmental Rates of Mouse Oocytes
Vitrification Fertilization Maturation Cryotop Developmental Rate Oocyte
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2015/11/9
Background: Oocyte cryopreservation is an important part of modern fertility treatment. The effect of vitrification on the fertilization and developmental rates of embryo is still a matter of debate.
The role of nitric oxide synthase isoforms in aged porcine oocytes
nitric oxide L-NAME aminoguanidine oocyte ageing pig
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2015/6/24
In the sphere of reproductive biotechnologies, the demand for sufficient numbers of high-quality oocytes is still increasing. In some cases, this obstacle is overcome by in vitro prolonged cultivation...
Effects of inhibiting nitric oxide synthase on cumulus expansion and nuclear maturation of sheep oocytes
sheep oocyte maturation nitric oxide L-NAME
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2015/6/9
Nitric oxide (NO) is a biological signaling molecule that plays a crucial role in oocyte maturation of mammalians. It is generated by the nitric oxide synthase (NOS) enzyme from l-arginine. Although t...
Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor
activation nitric oxide oocyte
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2015/6/8
Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor SNAP (2mM). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. ...
Production of Boer goat (Capra hircus) by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes
goat fibroblast clone
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2015/6/8
The present study reports a birth of goat clone produced by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes. The donors of somatic cells were fibrob...
Porcine oocytes matured in vitro develop in various ways if they are further cultivated. In our studies these oocytes were cultivated for 1 to 5 days (in vitro aging). During the 1st day of aging, mos...
Effects of genistein and genistin on in vitro maturation of pig oocytes
phytoestrogen isoflavone oocyte maturation pig estrogenic effect tyrosine protein kinase
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2015/6/5
The objective of the study was to verify the hypothesis that GEN (genistein – phytoestrogen and an inhibitor of tyrosine protein kinase – TPK) effects on pig oocyte maturation and cumular cell expansi...
The role of nitric oxide in parthenogenetic activation of pig oocytes: A review
oocyte parthenogenesis activation nitric oxide
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2015/6/4
Parthenogenetic activation of mammalian oocytes with artificial stimuli is commonly applied in various reproductive biotechniques, e.g. cloning using nuclear transfer. For this reason, many studies fo...
Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor
oocyte pig parthenogenesis activation calcium ionophore nitric oxide
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2015/6/4
Porcine oocytes matured in vitro were activated for parthenogenetic development using either calcium ionophore (50μM for 10 min) or nitric oxide donor SNAP (2mM for 23.5 hours). Protein kinase C (PKC)...
Parthenogenetic development of rabbit oocytes after electrical stimulation
rabbit oocytes electric pulses in vitro culture
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2015/6/2
The aim of this study was to determine the effect of electric pulses on the structural and functional condition of rabbit oocytes. The New Zealand White female rabbits at 3–5 months of age and at 3–4 ...
Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities
Brilliant Cresyl Blue Embryonic Development Subcellular Characteristics
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2016/5/19
The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogena...
Causes and consequences of maternal age-related aneuploidy in oocytes: a review
aneuploidy oocyte maternal age meiosis spindle assembly checkpoint
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2015/4/17
Although a positive correlation between aneuploidy and maternal age was first reported almost a century ago, the underlying mechanisms remain mostly unknown. Different hypotheses regarding age-related...
Expression of cyclin-dependent kinase inhibitors (CDKN1, CDKN5) in developmentally competent and incompetent porcine oocytes
Cdkn1 Cdkn5 oocyte developmental competence
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2015/4/16
Although several reports have been published regarding the cyclin-dependent kinases (Cdks) in developmentally competent mammalian oocytes, still little is known about their expression pattern in devel...
Maternal nutrition affects the composition of follicular fluid and transcript content in gilt oocytes
fatty acids BCB test pig EEF1A1 ATP5A1
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2015/4/15
Metabolomics focused on reproduction have been the subject of special interest in the past decade. Metabolite profiling provides detailed information on the status of follicular fluid and follicular c...