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Use of Lysozyme as a Feed Additive on In vitro Rumen Fermentation and Methane Emission
In vitro Lysozyme Methane Ruminant Volatile Fatty Acid
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2016/11/10
This study was conducted to determine the effect of lysozyme addition on in vitro rumen fermentation and to identify the lysozyme inclusion rate for abating methane (CH4) production. An in vitro rumin...
Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue
Yak Mammary Tissue Milk Lysozyme Recombinant Protein
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2016/5/19
Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp ...
Expression, Purification, and Characteristic of Tibetan Sheep Breast Lysozyme Using Pichia pastoris Expression System
Pichia pastoris Purification Secretory Expression Tibetan Sheep
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2016/5/16
A lysozyme gene from breast of Tibetan sheep was successfully expressed by secretion using a-factor signal sequence in the methylotrophic yeast, Pichia pastoris GS115. An expression yield and specific...
Sequence Analysis of Lysozyme C from the Scorpion Mesobuthus Eupeus Venom Glands Using Semi- Nested RT-PCR
C-type lysozyme Scorpion Mesobuthus eupeus Antimicrobial protein
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2015/9/24
Background: Lysozyme is an antimicrobial protein widely distributed among eukaryotes and prokaryotes and take part in protecting microbial infection. Here, we amplified cDNA of MesoLys-C, a c-type lys...
Equilibrium Clusters in Concentrated Lysozyme Protein Solutions
equilibrium clusters, salt-free lysozyme solutions, short-range attraction and weak long-range repulsion effective potential, scattering experiments.
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2011/9/9
We have studied the structure of salt-free lysozyme at 293 K and pH 7.8 using molecular simulations and experimental SAXS effective potentials between proteins at three volume fractions, f = 0.012, 0....
PCR-SSCP of Serum Lysozyme Gene (Exon-III) in Riverine Buffalo and Its Association with Lysozyme Activity and Somatic Cell Count
Polymorphism Lysozyme Gene SSCP Buffalo Association SCC Sequencing
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2016/5/5
Serum lysozyme gene is one of the important genes influencing the immune system as its product can cause lysis of bacterial cell wall by cleaving the peptidoglycan layer. The present investigation on ...
Effect of pH and Addition of Salt on the Adsorption Behavior of Lysozyme on Gold, Silica, and Titania Surfaces Observed by Quartz Crystal Microbalance with Dissipation Monitoring
Lysozyme Adsorption QCM-D
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2009/9/11
The adsorption behaviors of lysozyme on dentally related Au, SiO2, and TiO2 surfaces were investigated by a quartz crystal microbalance with dissipation monitoring (QCM-D) method. Frequency shifts ind...
Optimum Condition of High Pressure Treatment for the Preparation of Lysozyme-Dextran Complex Found by Random-Centroid Optimization
lysozyme-dextran complex high pressure random-centroid optimization
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2009/6/18
The lysozyme-dextran complex (LDC) was prepared in the liquid state using high pressure treatment. This method was able to shorten the reaction period for the preparation of LDC and to suppress the fo...
Production of Antimicrobial Films by Incorporation of Partially Purified Lysozyme into Biodegradable Films of Crude Exopolysaccharides Obtained from Aureobasidium pullulans Fermentation
lysozyme exopolysaccharides pullulan
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2009/3/16
Antimicrobial films were produced by incorporating partially purified lysozyme into films of crude exopolysaccharides (59 % pullulan) obtained from Aureobasidium pullulans fermentation. After film mak...
The pressure-temperature phase diagram of hen lysozyme at low pH
hen lysozyme high pressure fluorescence high pressure NMR thermodynamic stability on pressure-temperature axes free energy landscape
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2010/12/2
The equilibrium unfolding of hen lysozyme at pH 2 was studied as a function of pressure (0.1~700 MPa) and temperature (−10°C~50°C) using Trp fluorescence as monitor supplemented by variable pres...
Magnetic Cation Exchange Isolation of Lysozyme from Native Hen Egg White
hen egg white lysozyme magnetic separation
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2009/2/26
Two magnetic macroporous cellulose cation exchangers (Iontosorb MG CM 100 and Iontosorb MG SHP 100) were used for one-step isolation of lysozyme from native, undiluted hen egg white. Highly purified l...
Partial Purification of Hen Egg White Lysozyme by Ethanol Precipitation Method and Determination of the Thermal Stability of Its Lyophilized Form
Lysozyme hen egg white partial purification ethanol precipitation thermal stability
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2009/1/9
Lysozyme was partially purified from hen egg white by precipitation of non-lysozyme protein impurities during incubation in the presence of ethanol. The thermal stability of the obtained partially pur...
Transfer of Lysozyme Gene into indica Parents of Hybrid Rice by Backcrossing
hybrid rice lysozyme gene rice blast backcrossing breedingRice
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2009/1/7
A lysozyme gene resistant to rice blast was transferred from the donor transgenic japonica rice Zhonghua 9 (D2-1-2) into a sterile line Pei’ai 64S(PA 64S) and restorer line 9311 of the two-line hybrid...
The Effect of Mammary Gland Expression of Human Lysozyme on the Properties of Milk from Transgenic Mice
transgenic human lysozyme milk
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2008/3/23
Transgenic mice were used as model systems to evaluate the impact of human lysozyme expression in the mammary gland. We previously generated two lines of transgenic mice that express human lysozyme mR...
Lysozyme Activity in Buffalo Milk: Effect of Lactation Period, Parity, Mastitis, Season in India, pH and Milk Processing Heat Treatment
Lysozyme Buffalo Milk Bovine Milk Parity Mastitis
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2016/4/11
Lysozyme activity in buffalo milk in relation to the period of lactation, parity of animal, weather conditions and udder infections was studied. Effect of storage and heat processing of milk on lysozy...