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Quantification of apoptotic testicular germ cells in normal and methoxyacetic acid-treated mice as determined by flow cytometry
Spermatogenesis TUNEL
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2009/2/18
Several studies have reported the occurrence and significance of programmed cell death (apoptosis) of testicular germ cells in mammals. In those studies, apoptotic germ cells were identified by mo...
Effect of semen dilution on bovine sperm viability as determined by dual-DNA staining and flow cytometry
Sperm concentration cellular viability SYBR-14
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2009/2/17
Living and dead spermatozoa were examined for the effects of sperm concentration level on sperm viability. Semen was collected from two different bulls on each of four collection dates. A ninth bull...
Detection and quantitation of sperm-bound antibodies by flow cytometry of human semen
Spermatozoa antibody infertility
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2009/2/10
Detection of sperm surface antibodies is important for the diagnosis and treatment of infertility. The authors have investigated the methodologic aspects of flow cytometry (FCM) to detect sperm-bo...
Application of flow cytometry to studies on the human acrosome
flow cytometry lectin acrosome normooospermia acrosome reaction
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2009/2/6
This study describes the use of flow cytometry combined with specific labelling of the human sperm acrosome using a FITC-labelled plant lectin (Arachis hypogea agglutinin). Localization of the label...
DNA-flow cytometry of defined stages of rat seminiferous epithelium: effects of 3 Gy of high-energy X-irradiation
testis Sertoli germ cell
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2009/2/6
Testes of adult Sprague-Dawley rats were irradiated locally by 3 Gy of 4 MeV X-rays produced by a linear accelerator. This type and dose of radiation gives an even distribution through the testis an...
Correlation between clusterin-positive spermatozoa determined by flow cytometry in bull semen and fertility
Correlation clusterin-positive spermatozoa cytometry bull semen fertility
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2008/12/25
The objectives were to 1) develop a rapid and accurate method for detection of clusterin-positive spermatozoa (CPS) in bull semen and 2) determine the utility of incidence of CPS for prediction of f...
Measurement of intracellular calcium concentration and plasma membrane potential in human spermatozoa using flow cytometry
Measurement intracellular calcium concentration plasma membrane potential human spermatozoa
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2008/12/25
We report 2 novel approaches using flow cytometry to measure intracellular calcium concentration and plasma membrane potential in human spermatozoa. Both approaches have the potential to measure dif...
Characterizing the Glycocalyx of Poultry Spermatozoa：I. Identification and Distribution of Carbohydrate Residues Using Flow Cytometry and Epifluorescence Microscopy
Turkey chicken semen glycoconjugate lectin
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2008/12/24
The aim of the present work was to use a battery of lectins to 1) delineate the carbohydrate content of sperm glycocalyx in the turkey and chicken using flow cytometry analysis, and 2) evaluate the ...
Relationship Between Sperm Viability as Determined by Flow Cytometry and Nonreturn Rate of Dairy Bulls
Spermatozoa quality insemination fertility precision accuracy
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2008/12/23
A newly developed flow cytometric method for determination of sperm concentration and viability was tested in an insemination trial with cryopreserved bull sperm to establish the relationship betwee...