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Mechanism of LHRH-stimulated steroidogenesis in rat Leydig cells：lipoxygenase products of arachidonic acid may not be involved
LHRH-stimulated steroidogenesis rat Leydig cells lipoxygenase products arachidonic acid
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2009/2/23
Luteinizing hormone releasing hormone agonist, [(imBzl)-DHis6,Pro9,NEt]-LHRH (LHRH-A), caused a two to threefold increase in in vitro testosterone (T) secretion by rat Leydig cells. This LHRH-A-indu...
Uptake and release of ascorbic acid by rat Leydig cells in vitro
ascorbic acid rat Leydig cells
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2009/2/23
The mechanism by which ascorbic acid is transported into rat Leydig cells in vitro was investigated. Transport of [14C]ascorbic acid was temperature-dependent and inhibited in the absence of sodium ...
The effect of selective destruction and regeneration of rat Leydig cells on the intratesticular distribution of testosterone and morphology of the seminiferous epithelium
selective destruction regeneration rat Leydig cells intratesticular distribution
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2009/2/23
This study was designed to explore the relationship between the intratesticular distribution of testosterone and spermatogenesis by completely destroying the Leydig cells of mature male rats with in...
Evidence for the presence of androgen receptors in purified rat Leydig cells
androgen receptors purified rat Leydig cells
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2009/2/19
Leydig cells, purified on two sequential Percoll gradients to purities of 89 +/- 1%, were used to study the binding of 17-beta-hydroxy-17 alpha-methyl-estra-4,9,11-triene-3-one(3H-R1881). The accumu...
Effects of insulin-like growth factor-I on androgen production by highly purified pubertal and adult rat Leydig cells
Somatomedin-C development steroidogenesis
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2009/2/10
Leydig cells were isolated and purified from adult and midpubertal rats to study the effects of insulin-like growth factor-I (IGF-I) on steroidogenesis. Androgen production, as measured in Leydig ce...
Tumor necrosis factor and interleukin-1 stimulate testosterone secretion in adult male rat Leydig cells in vitro
umor necrosis factor interleukin-1 testis testosterone
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2009/2/6
The actions of two cytokines, tumor necrosis factor (TNF) and interleukin-1 (IL-1), on testosterone production by dispersed adult testis cells and purified Leydig cells in culture were studied. In o...
Initial characterization of factors from testicular fluid which alter in vitro androgen secretion by normal rat Leydig cells
testis testosterone seminiferous tubule
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2009/2/6
It is known that testicular interstitial fluid (TF) contains thermolabile factors that can alter in vitro production of androgens by the Leydig cells. The net stimulatory activity of this fluid incr...
Leydig cell cooperation in vitro：evidence for communication between adult rat Leydig cells
rat adult Leydig cells in vitro cooperation autocrine
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2009/1/5
In short-term incubations (32 C, 3 h) of purified adult rat Leydig cells, increasing the density from 5000 to 50,000 cells/16 mm diameter culture well caused a significant increase in human chorioni...
Protein kinase C increases 11beta-hydroxysteroid dehydrogenase oxidation and inhibits reduction in rat Leydig cells
Calcium calmodulin glucocorticoid action oxidoreductases
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2008/12/30
Glucocorticoid hormone controls Leydig cell steroidogenic function through a receptor-mediated mechanism. The enzyme 11beta-hydroxysteroid dehydrogenase (11betaHSD) plays an important role in Leydig...
Purification of rat leydig cells：increased yields after unit-gravity sedimentation of collagenase-dispersed interstitial cells
Clusters gap junctions macrophages
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2008/12/29
Procedures for purification of Leydig cells have facilitated studies of their regulatory biology. A multistep procedure that includes a filtration with nylon mesh (100-micron pore size) to separate ...
Initial predominance of the oxidative activity of type I 11beta-hydroxysteroid dehydrogenase in primary rat Leydig cells and transfected cell lines
Initial predominance oxidative activity 11beta-hydroxysteroid dehydrogenase Leydig cells transfected cell
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2008/12/25
Glucocorticoids suppress testosterone production in Leydig cells. The level of glucocorticoid action is set within the Leydig cell by the number of glucocorticoid receptors and by the activity of ...
Paracrine Modulation of Androgen Synthesis in Rat Leydig Cells by Nitric Oxide
Macrophage testosterone DNA gene array nitric oxide synthase S-nitrosoglutathione L-NG-nitro-arginine methyl ester L-NG-monomethyl-arginine
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2008/12/23
The free radical nitric oxide (NO), generated through the oxidation of L-arginine to L-citrulline by NO synthases (NOSs), has been shown to inhibit steroidogenic pathways. NOS isoforms are known...
Protein kinase C increases 11beta-hydroxysteroid dehydrogenase oxidation and inhibits reduction in rat Leydig cells
Protein kinase C 11beta-hydroxysteroid dehydrogenase oxidation inhibits rat Leydig cells
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2008/12/1
Glucocorticoid hormone controls Leydig cell steroidogenic function through a receptor-mediated mechanism. The enzyme 11beta-hydroxysteroid dehydrogenase (11betaHSD) plays an important role in Leyd...