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Effect of Mytomycin C Treatment on Feeder Cells and the Proliferation of Cultured Chicken Primordial Germ Cells Isolated from Embryonic Blood
chicken primordial germ cell culture feeder cell mytomycin C
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2009/8/3
Chicken primordial germ cells isolated from embryonic blood were cultured in vitro on feeder cells derived from the gonads of 7-day incubated embryos. Primordial germ cells were proliferated about 13 ...
Conservation of a Genetically Selected Broiler Line (42L)Using Cryopreserved Circulating Primordial Germ Cells (PGCS) Isolated by Filtration Method
Filtration method PGCs cryopreservation germ-line chimeras chicken
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2009/7/31
A genetically selected broiler line (42L) developed at the Pennsylvania State University, PA, USA was used in the present study. In Experiment 1, embryonic blood was collected from chick embryos at ...
The Migratory Ability of Gonadal Germ Cells in the Domestic Chicken
germ cells migratory ability germline chimera chicken
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2009/7/31
The migratory ability of germ cells collected from gonads at different stages of embryonic development was investigated in the domestic chicken.
In Experiment 1, primordial germ cells (PGCs) were col...
Effects of Ultraviolet Irradiation on the Migratory Ability of Primordial Germ Cells (PGCs) in the Domestic Chicken
UV irradiation PGCs Migratory ability embryo chicken
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2009/7/31
The effect of ultraviolet (UV) irradiation on the ability of primordial germ cells (PGCs) to migrate toward the gonadal ridge was investigated in the domestic chicken.
When PGCs were irradiated with ...
Conservation of a Threatened Indigenous Fowl (Kureko Dori) Using the Germline Chimeras Transplanted from Primordial Germ Cells
endangered species domestic fowl germline chimera primordial germ cells transplantation
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2009/7/30
This is the first successful report on producing the endangered domestic fowl, Kureko Dori (KD), which is a Kumamoto prefectural natural treasure, using germline chimeras transplanted from primordial ...
Cryopreservation of Primordial Germ Cells (PGCs) from White Leghorn Embryos Using Commercial Cryoprotectants
chicken cryoprotectants PGCs recovery-rate viability
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2009/7/30
Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the large size of their yolk structure. Cryopreservation of primordial...
Production of Inter-Genus Somatic Nuclear Transferred Gonadal Germ Cells (snt-GGCs) in Avian Species
chicken electrofusion gonadal germ cells quail somatic nuclear transfer
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2009/7/30
Interspecies or even inter-genus somatic nuclear transfer is considered to be an effective means for conserving a wide variety of avian genetic resources. However, somatic nuclear transfer offspring p...
Cryopreservation of Gonadal Germ Cells (GGCs) from the Domestic Chicken Using Vitrification
chicken cryopreservation gonadal germ cells migratory ability vitrification
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2009/7/29
We cryopreserved gonadal germ cells (GGCs) collected from 7-day-old chick embryos using the vitrification method. Gonadal germ cells were suspended in vitrification medium and plunged directly into li...
Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs
chicken chimera primordial germ cell quail soft x-ray
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2009/7/29
The generation of germline chimeras by the transfer of primordial germ cells (PGCs) requires incorporation of the PGCs of the donor into the gonadal tissue of the recipient embryo. We investigated the...
Effects of Busulfan Sustained-release Emulsion on Depletion and Repopulation of Primordial Germ Cells in Early Chicken Embryos
busulfan sustained-release emulsion early chicken embryos germline chimeras primordial germ cells
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2009/7/29
The current study aimed to determine the optimal time of busulfan (1,4-butanediol dimethanesulfonate) administration for the preparation of suitable recipient embryos to generate germline chimeras in ...
Preferential Migration of Transferred Primordial Germ Cells to Left Germinal Ridge of Recipient Embryos in Chickens
chicken germinal ridge migration primordial germ cell
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2009/7/29
Chicken primordial germ cells (PGCs) circulate in the bloodstream and migrate to germinal ridges.In order to assess the migration frequencies of PGCs to left or right germinal ridges, PGCs were transf...