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Covalent Self-labeling of Tagged Proteins with Chemical Fluorescent Dyes in BY-2 Cells and Arabidopsis Seedlings
BY-2 cells Arabidopsis SNAP-tag live cell imaging microtubules endocytosis PIN2 auxin transporter
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2023/12/21
Synthetic chemical fluorescent dyes promise to be useful for many applications in biology. Covalent, targeted labeling, such as with a SNAP-tag, uses synthetic dyes to label specific proteins in vivo ...
Protein kinase sensors: an overview of new designs for visualizing kinase dynamics in single plant cells
plant cells protein kinase sensors kinase dynamics signaling networks SnRK2
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2023/12/1
Protein kinase dynamics play key roles in regulation of cell differentiation, growth, development and in diverse cell signaling networks. Protein kinase sensors enable visualization of protein kinase ...
Cross-talk between clathrin-dependent post-Golgi trafficking and clathrin-mediated endocytosis in Arabidopsis root cells
Adaptor protein Arabidopsis TPLATE clathrin endocytosis exocytosis
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2023/12/1
Coupling of post-Golgi and endocytic membrane transport ensures that the flow of materials to/from the plasma membrane (PM) is properly balanced. The mechanisms underlying the coordinated trafficking ...
Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells
Single-cell transcriptomics photosynthetic Arabidopsis thaliana high-throughput transcriptome metabolic stress-related
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2023/11/30
As the main photosynthetic instruments of vascular plants, leaves are crucial and complex plant organs. A strict organization of leaf mesophyll and epidermal cell layers orchestrates photosynthesis an...