搜索结果: 1-15 共查到“作物学 Rubisco”相关记录17条 . 查询时间(0.088 秒)
华中农业大学在植物Rubisco遗传改良方面取得新进展
华中农业大学 植物 Rubisco 遗传改良
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2023/2/24
近日，华中农业大学生命科学技术学院、作物遗传改良全国重点实验林拥军教授团队联合英国利物浦大学刘鲁宁教授科研团队在利用细菌快速Rubisco优化高等植物光合作用领域取得新进展。相关研究结果以“Producing fast and active Rubisco in tobacco to enhance photosynthesis”为题在线发表在国际期刊The Plant Cell。
Endeavours of RuBisCO small subunit promoter as a tool of green tissue specific expression
constitutive expression rbcS promoter spatio-temporal expression
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2014/2/25
Transcriptomics has the potential to rapidly increase our knowledge of spatial and temporal gene expression and contributes to the characterization of new promoters for research and development. The s...
芥菜Rubisco小亚基的基因克隆及其在芜菁花叶病毒侵染后的表达分析
芥菜 Rubisco小亚基 抗病性 叶绿素荧光 TuMV
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2011/11/25
以耐病性叶用芥菜为材料,克隆和分析了芥菜Rubicso小亚基的基因序列,研究了芜菁花叶病毒(Turnip mosaic virus,TuMV)侵染对Rubicso小亚基基因表达的影响。结果显示:Rubicso小亚基基因全长为842bp,其开放阅读框(ORF)可编码181个氨基酸,其中包括2个α螺旋及4个β折叠二级结构,并含有一些高度保守的氨基酸残基。接种TuMV后,随着上部第3片真叶中外壳蛋白基因...
Rubisco活化酶(RCA)是一种可激活光合作用关键酶Rubisco的伴侣蛋白，可通过对Rubisco的活性调节决定植物的碳同化效率。为了研究玉米ZmRCA1的多态性，本研究参考GenBank中编码玉米Rubisco活化酶的ZmRCA1基因组序列设计引物对玉米微核心种质的95份自交系的ZmRCA1进行测序，获得长约1 680 bp的基因组序列。多态分析表明，在1 680 bp的区间内共发现22个...
水稻叶片自然衰老过程中Rubisco大亚基的含量变化
水稻 叶绿素b 突变体 衰老 光合放氧 1,5二磷酸核酮糖羧化酶/加氧酶
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2009/9/23
以水稻低叶绿素b突变体及其野生型（镇恢249）为材料，研究了水稻第5叶叶片自然衰老过程中光合放氧、Rubisco大亚基含量变化以及对胰蛋白酶敏感性的变化，并通过喷施不同的抗氧化剂以及氧化剂研究其对Rubisco大亚基含量变化的影响。结果表明，叶片全展后随着衰老进程，突变体与野生型光合放氧速率、Rubisco大亚基相对含量呈下降趋势，抗氧化剂可以不同程度地缓解Rubisco大亚基含量的下降，而氧化剂...
Photosynthetic Control Factors in a Single Leaf of Sweet Potato, Ipomoea batatas Lam : 3. Estimation of in vivo rubisco activity from the CO2 exchange rate of a peeled leaf
CO2 exchange rate Ipomoea batatas Lam Leaf epidermis peeling Rubisco activity
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2009/7/30
Rubisco (ribulose 1, 5-bisphosphate carboxylase/oxygenase) activity is the prime determinant for CO2 exchang rate (CER) at the beginning of the photosynthetic reaction, and hence the rubisco activity ...
Relationships between Photosynthetic Activity and the Amounts of Rubisco Activase and Rubisco in Rice Leaves from Emergence through Senescence
Aging Oryza sativa L Photosynthesis Rice Rubisco Rubisco activase Senescence
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2009/7/27
Photosynthetic activity determined as O2 evolution (OER) and the amounts of Rubisco activase, Rubisco, total soluble protein and chlorophyll were investigated in the 10th leaf of rice, Oryza sativa L....
Correlation of Leaf Nitrogen,Chlorophyll and Rubisco Contents with Photosynthesis in a Supernodulating Soybean Genotype Sakukei 4
Chlorophyll Glycine max Leaf nitrogen Photosynthesis Rubisco Soybean Supernodulation
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2009/7/24
Soybean requires more nitrogen (N) than gramineous crops because it accumulates a large amount of N in seeds, and its photosynthetic rate per leaf N is low. The supernodulating genotype Sakukei 4 has...
Gene Expression and Accumulation of Rubisco in Bundle Sheath and Mesophyll Cells during Leaf Development and Senescence in Rice,a C3 Plant
Bundle sheath C3 photosynthesis Chlorophyll fluorescence Immuno-localization In situ hybridization Leaf development Leaf senescence Mesophyll
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2009/7/23
Gene expression of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (rbcL) and small subunit (rbcS) in bundle sheath and mesophyll cells of rice, a C3 plant, was examined during...
Screening of High kcat Rubisco among Poaceae for Improvement of Photosynthetic CO2 Assimilation in Rice
kcat Km Oryza sativa L. Photosynthesis Rice Rubisco
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2009/7/21
The activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a major limitation of photosynthetic CO2 assimilation in C3 plants. In order to find useful Rubisco for improvement of phot...
The QTL Analysis of RuBisCO in Flag Leaves and Non-Structural Carbohydrates in Leaf Sheaths of Rice Using Chromosome Segment Substitution Lines and Backcross Progeny F2 Populations
Backcross progeny Chromosome segment substitution lines (CSSLs) Leaf sheath Non-structural carbohydrate (NSC) QTL Rice (Oryza sativa L.) RuBisCO
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2009/7/21
In rice (Oryza sativa L.), the maintenance of high photosynthetic rate of flag leaves and the carbon remobilization from leaf sheaths after heading is a critical physiological component affecting the ...
由核基因编码的叶绿体前体蛋白能否正确定向和转运到叶绿体，对叶绿体的功能十分重要。采用酵母双杂交方法，筛选豌豆的cDNA表达文库，分析检测与Rubisco小亚基前体蛋白（preSSU）相互作用并参与其调控的蛋白。结果表明，共有6种不同类型的蛋白与preSSU互作，分别为组氨酸蛋白激酶基因、转运蛋白复合体、转录和翻译相关蛋白及复合体、信号传导相关基因和光合合成相关基因。通过与相关蛋白的相互作用，pre...
水稻T-DNA插入突变体库中Rubisco抗氧化胁迫株系的筛选
水稻突变体库 Rubisco小亚基和大亚基含量 氧化胁迫 筛选
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2008/1/8
以水稻(Oryza sativa L. subsp. japonica)品种“中花11”构建的T-DNA插入突变体库为材料，用甲基紫精诱导叶绿体产生活性氧，通过SDS-PAGE电泳分析Rubisco含量相对于野生型的变化，筛选耐氧化胁迫的株系。结果表明，与对照相比，突变体材料各个株系叶片中的Rubisco小亚基的相对含量有较大差异，说明这种筛选Rubisco突变体的方法是可行的，且从中找到了两株R...
Rubisco活化酶基因反义表达载体的构建与水稻的遗传转化
水稻 Rubisco活化酶 反义 载体构建 遗传转化
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2008/1/6
摘要利用pGEM-T easy 、pBluescript KS+获得了RCA基因两端可以引入与双元表达载体pCAMBIA1301多克隆位点相匹配的单酶切位点，将该片段连接到pCAMBIA1301载体，构建了RCA基因的反义植物表达载体pCAMR02。通过电激法将该载体导入根癌农杆菌EHA105菌株中，以根癌农杆菌介导的方法，将反义载体pCAMR02转化粳稻品种中花11，获得了抗潮霉素的再生植株，经...
RUBISCO, Nitrate Reductase and IAA Oxidase Activity of Rice Genotypes through Aerobic Cultivation
Aerobic rice physiological traits enzyme activity yield components yield
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2010/11/10
The rice genotypes viz., PMK 3, ASD 16, MDU 3, MDU 5, CO 47 and RM 96019 were evaluated under aerobic condition during kharif 2005 to study the physiological traits and yield attributes.
The paramete...