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用农杆菌介导法将AtNHX1基因导入菊苣中，共获得４２株卡那霉素（Ｋａｎ）抗性再生植株。经过ＰＣＲ 检测、Ｓｏｕｔｈｅｒｎ杂交和ＲＴ－ＰＣＲ 检测表明，AtNHX1基因已成功整合到菊苣基因组中，并且能够正常转录。野生型和转基因植株诱发的愈伤组织进行耐盐生长试验，结果显示，相同盐胁迫条件下，转基因愈伤组织的相对生长率显著高于野生型愈伤组织。施加梯度ＮａＣｌ胁迫后，植株叶片Ｋ＋ 和Ｎａ＋ 含量测定结果...
Ectopic Expression of AtNHX1 in Cotton (Gossypium hirsutum L.) Increases Proline Content and Enhances Photosynthesis under Salt Stress Conditions
Ectopic Expression AtNHX1 Cotton Proline Content Photosynthesis Salt Stress Conditions
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2008/12/10
Overexpression of the Arabidopsis gene AtNHX1 that codes for a vacuolar Na+/H+ antiporter in transgenic cotton significantly improved tolerance to 200 mM NaCl as measured by increases in plant height,...