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Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
recombinant antigen ELISA immunoblo
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2015/1/20
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal bindi...
硝酸镧引起Escherichia coli B代谢过程热爆发及其机理研究
硝酸镧 Escherichia coli Escherichia coli 微量热 热爆发 氧化磷酸化
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2007/12/24
摘要 采用微量热法研究了硝酸镧对Escherichia coli B生长代谢过程的影响, 发现高浓度硝酸镧引起E. coli B热谱图出现异常变化: 生长速率常数k值增大、产热峰显著升高和总发热量异常增加. 当硝酸镧浓度为300和500 mg/L时, 培养物在培养过程的总发热量分别是正常条件下的3.89和2.54倍. 用生物学方法对细胞存活率和生物量进行测定结果表明, 细胞在高浓度硝酸镧条件下增殖...
Biosynthesis and characterization of 4-flurotryptophan-labeled Escherichia coli arginyl-tRNA synthetase
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2007/7/28
期刊信息
篇名
Biosynthesis and characterization of 4-flurotryptophan-labeled Escherichia coli arginyl-tRNA synthetase
语种
英文
撰写或编译
撰写
作者
Qing-shuo Zhang,Li Shen,En-Duo Wang(通讯作者),Ying-lai Wang
第一作者单位
中科院上海生化...
A Comparative Study on the Recovery of EcoRI Endonuclease from Two Different Genetically Modified Strains of Escherichia coli
EcoRI endonuclease recombinant E. coli purification enzyme recovery
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2010/6/27
A laboratory scale procedure developed for the purification of EcoRI restriction endonuclease was applied to two different Escherichia coli} strains, E. coli 294 and E. coli M5248, which are genetical...
Optimization of Starting Time and Period of Induction and Inducer Concentration in the Production of the Restriction Enzyme EcoRI from Recombinant Escherichia coli 294
EcoRI restriction enzyme induction enzyme purification
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2010/6/30
Induction parameters including inducer concentration, period of induction and the cell concentration at which inducer is to be added to the fermentation broth were optimized in order to increase the y...