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DNA extraction from human saliva deposited on skin and its use in forensic identification procedures
DNA Forensic dentistry Saliva
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2009/10/20
Saliva is usually deposited in bite marks found in many homicides, assault and other criminal cases. In the present study, saliva obtained from volunteers was deposited on skin and recovered for DNA e...
Study of DNA damage induced by dental bleaching agents in vitro
CHO cells Comet assay Tooth bleaching Mutagenicity tests
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2009/10/19
Dental bleaching is a simple and conservative procedure for aesthetic restoration of vital and non-vital discolored teeth. Nevertheless, a number of studies have demonstrated the risk of tissue damage...
樊明文小组“防龋DNA疫苗”研制获重大突破
樊明文 防龋DNA疫苗
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2009/9/21
我国5岁儿童患龋齿病率为64%，成年人达88%，而老年人几乎为100%！现对这样的现实，能否有一种疫苗让人们不再患龋齿呢？有！
Global Gene Expression Analyses of Mouse Fibroblast L929 Cells Exposed to IC50 MMA by DNA Microarray and Confirmation of Four Detoxification Genes' Expression by Real-time PCR
MMA cDNA microarray Gene expression
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2009/9/14
Methyl methacrylate (MMA) is the main component of methyl methacrylic resin, which is widely used in dentistry. Previous studies have investigated whether MMA has any adverse effects on growth and gen...
Drug Binding and Releasing Characteristics of DNA/Lipid/PLGA Film
Intercalation DDS DNA/lipid/PLGA film
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2009/9/14
We evaluated the blended compound of DNA/lipid complexes and PLGA (poly(D,L-lactide-co-glycolide)) as a carrier material for drug delivery system (DDS). Transparent, self-standing DNA/lipid/PLGA films...
线粒体DNA 突变与头颈肿瘤发生的研究进展
头颈肿瘤 DNA 线粒体 突变
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2014/3/19
线粒体DNA 是核外唯一的遗传物质，较核DNA 更易发生突变。在头颈肿瘤中，线粒体DNA 所存在的点突变、片段缺失和微卫星不稳定性等现象可能与头颈肿瘤的发生相关，故线粒体DNA 有望成为一种检测头颈肿瘤的新的生物标志物。笔者下面就线粒体DNA 的结构和功能特点、头颈肿瘤中线粒体DNA 的突变、线粒体DNA 突变与头颈肿瘤发生的关系、线粒体DNA 突变的临床价值等研究进展作一综述。
目的与方法:本文对54 例口腔白斑患者(均为吸烟者) 进行了口腔脱落粘膜细胞微核和外周血淋巴细胞微核及染色体畸变率检测。结果:吸烟的口腔白斑患者口腔脱落粘膜细胞微核细胞率和外周血淋巴细胞微核细胞率及染色体畸变率均显著高于正常人,且口腔白斑病损区的脱落粘膜细胞微核细胞率的变化与口腔白斑病变程度相关,正常区粘膜脱落细胞微核细胞率的高低与外周血淋巴细胞微核细胞率呈正相关。结论:结果表明,口腔白斑病损区脱...
Detection of Microsatellite Alterations in Plasma DNA of Malignant Mucosal Melanoma Using Whole Genome Amplification
Free-circulating DNA Plasma DNA Loss of heterozygosity
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2010/12/1
Malignant mucosal melanoma (MMM) still has the poorest prognosis. There is a paucity of molecular biological studies on MMM of the head and neck. We investigated free-circulating DNA microsatellites w...
Viability of the Cariostat® medium as a source of DNA for further analysis through polymerase chain reaction
Cariostat DNA Streptococcus mutans PCR
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2008/4/16
This simulation study was carried out to analyze and evaluate Cariostat-inoculated samples through a conventional PCR protocol specific for Streptococcus mutans strains and also for the purpose of est...
Detection of Tumor DNA in Plasma Using Whole Genome Amplification
Malignant melanoma, Adenoid cystic carcinoma Loss of heterozygosity (LOH) Plasma or serum DNA Circulating tumor DNA
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2010/1/20
Altered microsatellite DNA in the blood of cancer patients may provide a novel means for tumor detection. Such alterations are a major characteristic of many types of tumor especially those associated...
Detection of Tumor DNA in Plasma Using Whole Genome Amplification
Malignant melanoma Adenoid cystic carcinoma
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2010/12/1
Altered microsatellite DNA in the blood of cancer patients may provide a novel means for tumor detection. Such alterations are a major characteristic of many types of tumor especially those associated...
Identification of Unknown Body Using DNA Analysis and Dental Characteristics in Chest X-ray Photograph
Personal identification Skeletonized body DNA analysis Mitochondrial DNA Dental identification
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2010/1/21
An unknown skeletonized body was identified by DNA analysis and dental information. The body had already been cremated when a candidate for the unknown body was proposed. Therefore, for DNA analysis w...
Polymorphism of LPL Locus in Japanese and Comparison of PCR Amplification Efficiency from Degraded DNA between LPL Locus and the D21S11
Short tandem repeat (STR) LPL Japanese population D21S11 Degraded DNA
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2010/1/21
The short tandem repeat (STR) polymorphism of the lipoprotein lipase (LPL) locus was amplified by PCR and analyzed using denaturing polyacrylamide gel electrophoresis followed by silver staining. Amon...
Identification of Unknown Body Using DNA Analysis and Dental Characteristics in Chest X-ray Photograph
Personal identification Skeletonized body DNA analysis
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2010/12/1
An unknown skeletonized body was identified by DNA analysis and dental information. The body had already been cremated when a candidate for the unknown body was proposed. Therefore, for DNA analysis w...
Polymorphism of LPL Locus in Japanese and Comparison of PCR Amplification Efficiency from Degraded DNA between LPL Locus and the D21S11
Short tandem repeat LPL Japanese population
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2010/12/1
The short tandem repeat (STR) polymorphism of the lipoprotein lipase (LPL) locus was amplified by PCR and analyzed using denaturing polyacrylamide gel electrophoresis followed by silver staining. Amon...