搜索结果: 31-45 共查到“知识库 植物检疫学”相关记录159条 . 查询时间(1.378 秒)
Detection of Clavibacter michiganensis subsp. sepedonicus in daughter tubers of volunteer potato plants
potato Clavibacter michiganensis subsp. sepedonicus bacterial ring rot potato volunteer plants incidence of occurrence DAS ELISA Czech Republic
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2015/3/1
Daughter tubers of volunteer potatoes were tested for their ability to maintain Clavibacter michiganensis subsp. sepedonicus (Cms). In different areas of the CR, volunteer potatoes were searched for i...
Fusarium spp. In wheat grain in the Czech Republic analysed by PCR method
Fusarium spp. PCR detection wheat
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2015/3/1
The frequency of occurrence of four Fusarium spp. on wheat in the Moravia region, Czech Republic, was determined by polymerase chain reaction (PCR). Grain samples were collected during 2003–2006 at gr...
Reaction of winter wheat cultivars to common bunt Tilletia tritici (Bjerk.) Wint. and T. laevis Kühn
common bunt wheat resistance
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2015/3/1
Fifteen registered winter wheat cultivars were tested for reaction to common bunt in 2-years trials. A mixture of seven Czech proveniences of common bunt was used for inoculation. Cvs Globus and Bill ...
First report of potato stolbur phytoplasma in hemipterans in southern Moravia − Short Communication
stolbur Hyalesthes obsoletus vector PCR
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2015/3/1
In 2005, the first screening tests to confirm the presence of potato stolbur phytoplasma in hemipterans by the PCR method were carried out to determine the spectrum of possible vector species for furt...
Identification of agricultural soils suitable for afforestation in the Czech Republic using a soil database
soil survey Evaluated Soil Ecological Units soil quality climatic change water retention
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2015/5/6
Afforestation of agricultural lands may be a positive action in many cases. Especially, it is a beneficial feature for waterlogged soils, stony soils or for soils which are less fertile for any other ...
番茄细菌性叶斑病菌(Pseudomonas syringae pv. tomato, Pst)是我国进境植物检疫性有害生物, 可随种子进行远距离传播。快速简便的检测对于防止该病害的扩散传播具有重要意义。依据番茄细菌性叶斑病菌的hrpZPst 基因序列, 设计并筛选出特异性扩增引物, 建立了番茄细菌性叶斑病菌的重组酶聚合酶等温扩增(RPA)检测方法。该方法可从10种不同的植物病原细菌中特异性地检测到...
对汕头口岸自2002年以来从美国、巴西、阿根廷和乌拉圭进口大豆检疫疫情情况进行了综合分析。结果表明：检获一类危险性病害有大豆疫霉病菌和小麦矮腥黑穗病菌；对检获杂草进行分科统计分析，检疫性杂草有8个科26种，一般性杂草有27个科247种。并对各原产地检获疫情情况与其他口岸进行比较分析，寻找汕头口岸检获疫情的差距和进步，认为通过登轮查验、过筛检疫、复合取样、收集疫情载体、定期监测和后续一些检疫措施可以...
为分离筛选具有毒死蜱降解特性的植物内生菌,从农药厂废液池旁采集小飞蓬植物样本,经表面消毒后研磨提取植物汁液,通过以毒死蜱作为单一碳源的无机盐培养基(MSM)进行连续5代培养筛选,获得一株植物内生细菌XFP-gy,经生理生化试验及16SrDNA同源性比对分析,初步鉴定该菌属阪崎克罗诺杆菌属(Cronobacter sp.)。将菌株XFP-gy在以毒死蜱(初始质量浓度为20 mg/L)为单一碳源的MS...
烟草内生细菌YN201448的定殖能力研究
解淀粉芽孢杆菌 绿色荧光蛋白 拮抗作用 自然转化
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2015/9/8
为测定烟草内生细菌YN201448的定殖能力,通过自然转化法将携带绿色荧光蛋白基因的质粒pGFP4412成功导入YN201448细胞中,获得了GFP标记菌株48-pGFP。48-pGFP能稳定表达GFP蛋白,且对5种病原真菌的拮抗能力与野生型YN201448的相同。用48-pGFP菌液浸泡烟草种子和浇灌烟草苗后,在烟草的根、茎、叶等组织中都能检测到标记菌,其定殖数量分布为根 > 茎 > 叶;同时标...
转基因大豆GTS40-3-2成分现场可视化检测方法的建立
转基因大豆GTS40-3-2 转化体特异性 环状等温扩增技术 现场可视化检测
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2015/11/12
转基因大豆GTS40-3-2转化体是种植面积最广的转基因大豆品种，根据GTS40-3-2转化体特异性序列设计引物，应用可视化环介导等温扩增技术，对其进行快速扩增及可视化判断；同时建立转基因成分人工污染的食品模型，比较了LAMP法与定性PCR方法检测的敏感性。结果表明：该方法仅对GTS40-3-2转化体产生特异性扩增，灵敏度高达0.001%。所建立的GTS40-3-2转化体特异性现场可视化筛查方法具...
大豆品种（系）对不同疫霉菌生理小种的抗性研究
大豆 疫霉菌 抗性鉴定
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2015/11/12
对分布于黑龙江省各地区的60个大豆疫霉病菌株进行鉴定，初步鉴定为7个生理小种，选取黑龙江省优势小种1号、3号、9号为病原对208个国内外大豆品种（系）的抗性水平进行鉴定，以期获得抗疫霉根腐病的品种和种质资源。结果表明：208个大豆品种（系）中抗病品种比例较大，东北地区的大豆品种对疫霉根腐病1号、3号小种的抗性明显优于国外品种，而对9号生理小种则感病品种数量多于国外品种。
高效液相色谱-蒸发光散射检测器检测24-表芸苔素内酯原药含量
24-表芸苔素内酯 高效液相色谱 蒸发光散射检测器 定量检测
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2016/3/18
建立了高效液相色谱-蒸发光散射检测器（HPLC-ELSD）检测24-表芸苔素内酯原药含量的分析方法。在优化的ELSD条件（漂移管温度50 ℃、增益值10、辅助气压力350 kPa）下，采用Eclipse XDB C18色谱柱（250 mm×4.6 mm，5 μm），以V（乙腈）：V（水）=55：45等度洗脱、流速为1.0 mL/min、柱温30 ℃时，24-表芸苔素内酯原药中的有效成分和其他组分在...
Hyparrhenia variabilis and Hyparrhenia cymbaria (Poaceae):New for the Americas,Successful in Mexico
Invasive grasses invasive plants Jalisco Michoacán Morelos phytosanitary controls new record
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2015/12/26
Hyparrhenia cymbaria (boat thatching grass, ipopo grass) and Hyparrhenia variabilis (no common name), robust African savanna grasses with complex taxonomies, have not yet been reported for the America...
建立了蔬菜中烯肟菌胺残留量的固相萃取-气相色谱-质谱联用（SPE-GC-MS）分析方法；对烯肟菌胺的裂解机理进行了探讨；确定了测试分析的定性离子和定量离子。样品采用乙酸乙酯高速分散提取，以无水硫酸镁、硅胶和石墨化炭黑（GCB）为混合填料进行固相萃取净化，气相色谱-质谱联用仪在选择离子扫描（SIM）模式下进行检测，基质匹配标准溶液外标法定量。结果显示：烯肟菌胺在0.02~1 mg/L内，标准溶液的峰...